The goal of this study was to study the distribution of potential habitat markers (Sr/Ca, Ba/Ca, Mn/Ca and Li/Ca) in water from the Paranaguá Estuarine Complex (Brazil) and to study habitat use patterns of Cathorops spixii through ontogeny employing otolith microchemistry. Fish were caught from three sampling sites while water samples were collected at eight stations covering a salinity range from 4.5–33. Elemental concentrations in otolith and water were determined by LA-ICP-MS and ICP-MS, respectively. When the relationship between salinity and elements or ratios in water was studied, significant positive relationships were found for Sr, Li, Ca, Sr/Ca, and negative for Ba, Mn, Ba/Ca and Mn/Ca (P < 0.05). No relationship was observed between water Li/Ca and salinity. A significant positive correlation was found between otolith edge Sr/Ca and salinity (r = 0.63; P < 0.05), positioning this ratio as the best natural tag for reconstructing environmental histories of C. spixii. Change point analysis (CPA) based on otolith Sr/Ca signature through ontogeny revealed potential migrations between environments with different salinity. According to CPA, the number of displacements among different salinities ranged from 3–9 (6.1 ± 1.9), suggesting high plasticity in the migratory patterns. Ba/Ca, Li/Ca and Mn/Ca peaks were observed on the outer margin of the primordium, and could be influenced by physiological, environmental and maternal factors.

中文翻译：

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来自耳石化学的新热带河口自然标志物的空间环境变异性和Cathorops spixii的栖息地利用

本研究的目的是研究来自巴拉那瓜河口复合体（巴西）的水中潜在栖息地标记（Sr/Ca、Ba/Ca、Mn/Ca 和 Li/Ca）的分布，并研究栖息地利用模式刺槐通过使用耳石微化学的个体发育。鱼是从三个采样点捕获的，而水样是在八个站点收集的，盐度范围为 4.5-33。耳石和水中的元素浓度分别通过 LA-ICP-MS 和 ICP-MS 测定。当研究盐度与水中元素或比例的关系时，发现Sr、Li、Ca、Sr/Ca呈显着正相关，而Ba、Mn、Ba/Ca和Mn/Ca呈负相关。磷< 0.05)。没有观察到水 Li/Ca 和盐度之间的关系。耳石边缘 Sr/Ca 与盐度呈显着正相关（r= 0.63;磷< 0.05），将该比率定位为重建环境历史的最佳自然标签C. spixii. 通过个体发育基于耳石 Sr/Ca 特征的变化点分析 (CPA) 揭示了不同盐度环境之间的潜在迁移。根据 CPA，不同盐度之间的位移数量范围为 3-9（6.1 ± 1.9），表明迁移模式具有高可塑性。在原基外缘观察到Ba/Ca、Li/Ca和Mn/Ca峰，可能受生理、环境和母体因素的影响。