Abstract

In the present work, persistence and degradation of cyantraniliprole, a leading anthranilic diamide pesticide against sucking insect pests, in inceptisol soil has been studied. Effect of various factors like light source (UV-light, sunlight and dark), temperature (5, 25 and 37 °C), moisture regime (dry, field capacity and submerged) and carbon dioxide level (415 and 625 µL L⁻¹) on dissipation was also evaluated. Formation of IN-J9Z38, a major metabolite of cyantraniliprole, was monitored in different treatments. Samples were processed as per QuEChERS method and analysis was done by HPLC-PDA. Matrix matched calibration curve, prepared in the range 0.001–10 mg L^–1, indicated very good linearity (R² > 0.99) and sensitivity (instrument limit of detection 0.001 mg L^–1). Cyantraniliprole residues dissipated with time and 47.7%–98.8% dissipation was recorded on 90th day in different treatments. Minimum half-life of cyantraniliprole, i.e. 8.7 days was recorded at elevated CO₂ level (625 ± 5 µL L⁻¹) while its maximum half-life of 86.6 days was recorded at 5 °C. Metabolite IN-J9Z38 started forming from zero-day and reached the maximum on 15th to 60th day. Microbial biomass carbon, as an index of microbial activity of soil, correlated well with the degradation of cyantraniliprole.

摘要

在目前的工作中，已经研究了氰基硅腈（一种抗吸虫害的领先的邻氨基苯甲酰胺酰胺农药）在Inceptisol土壤中的持久性和降解性。各种因素的影响，例如光源（紫外线，日光和黑暗），温度（5、25和37°C），水分状况（干燥，野外处理能力和浸没状态）和二氧化碳水平（415和625 µL L ^-1）的耗散也进行了评估。在不同处理中监测了IN-J9Z38的形成，这是氰基腈的主要代谢物。根据QuEChERS方法处理样品，并通过HPLC-PDA进行分析。在0.001–10 mg L ^–1范围内制备的基质匹配校准曲线表明，线性非常好（R ²> 0.99）和灵敏度（检测限0.001 mg L ^–1）。在不同处理的第90天，氰基氰化物残留随时间消散，消散记录为47.7％–98.8％。在升高的CO ₂水平（625±5 µL L ^-1）下，氰基腈的最小半衰期为8.7天，而在5°C时的最大半衰期为86.6天。代谢物IN-J9Z38从零天开始形成，并在第15天到60天达到最大值。微生物生物量碳作为土壤微生物活性的指标，与氰基氰基丙烯腈的降解有很好的相关性。