ABSTRACT

The present work aimed to give an economical destiny to the produced water, a residue generated by the oil and gas industry by means of producing bioactives such as xanthan gum and ramnolipid. These compounds are often used in combination during enhanced oil recovery strategies. On the other hand, reports on co-culture of bacterial strains that are responsible for their production are rare. This research shows a factorial design method associated with surface response analysis to optimize carbon sources, sucrose and crude glycerin, and fermentation agents for culturing Xanthomonas campestris and Pseudomonas aeruginosa using the described conditions. After the critical point validation resulting in xanthan and ramnolipid production of 8.69 and 4.80 g L⁻¹, quality tests showed an apparent viscosity of 1006 cP with an emulsifying activity abouve 50% for 94 h.

摘要

目前的工作旨在使采出水具有经济意义，采出水是石油和天然气工业通过生产黄原胶和雷姆诺脂等生物活性物质而产生的残留物。这些化合物通常在提高采收率策略中组合使用。另一方面，关于产生它们的细菌菌株共培养的报道很少。这项研究显示了一种与表面响应分析相关的因子设计方法，以优化碳源、蔗糖和粗甘油，以及使用所述条件培养黄单胞菌和铜绿假单胞菌的发酵剂。经过临界点验证后，黄原胶和 ramnolipid 产量分别为 8.69 和 4.80 g L ^-1，质量测试显示表观粘度为 1006 cP，乳化活性高于 50%，持续 94 小时。