Introduction: Cow’s milk allergy (CMA) is one of the most common food allergies especially early in life. A mixture of nondigestible short-chain galacto-oligosaccharides, long-chain fructo-oligosaccharides, and pectin-derived acidic-oligosaccharides (GFA) may reduce allergy development and allergic symptoms in murine CMA. Recently, vitamin D (VitD) has been suggested to have beneficial effects in reducing allergy as well. Objective: In this study, the immune modulatory effect on allergy prevention using the combination of GFA and VitD was investigated. Methods: Female C3H/HeOuJ mice were fed a control or GFA-containing diet with depleted, standard (1,000 IU/kg), or supplemented (5,000 IU/kg) VitD content for 2 weeks before and during whey sensitization (n = 10–15). Mice were sensitized 5 times intragastrically with PBS as a control, whey as cow’s milk allergen, and/or cholera toxin as adjuvant on a weekly interval. One week after the last sensitization, mice were intradermally challenged in both ear pinnae and orally with whey, subsequently the acute allergic skin response and shock symptoms were measured. After 18 h, terminal blood samples, mesenteric lymph nodes, and spleens were collected. Whey-specific immunoglobulin (Ig) E and IgG1 levels were measured by means of ELISA. T cell subsets and dendritic cells (DCs) were studied using flow cytometry. Results: Additional VitD supplementation did not lower the allergic symptoms compared to the standard VitD diet. CMA mice fed the GFA diet supplemented with VitD (GFA VitD⁺) significantly decreased the acute allergic skin response of whey sensitized mice when compared to the CMA mice fed VitD (VitD⁺) group (p #x3c; 0.05). The effect of GFA was not improved by extra VitD supplementation even though the CMA mice fed the GFA VitD⁺ diet had a significantly increased percentage of CD103⁺ DCs compared to the VitD⁺ group (p #x3c; 0.05). The VitD-deprived mice showed a high percentage of severe shock and many reached the humane endpoint; therefore, these groups were not further analyzed. Conclusions: High-dose VitD supplementation in mice does not protect against CMA development in the presence or absence of GFA.
Int Arch Allergy Immunol

中文翻译：

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无论是否存在不可消化的低聚糖，膳食中的维生素D补充剂都无法有效地预防小鼠牛奶过敏。

简介：牛奶过敏（CMA）是最常见的食物过敏之一，尤其是在生命早期。不可消化的短链低聚半乳糖，长链低聚果糖和果胶衍生的酸性低聚糖（GFA）的混合物可减少鼠类CMA的变态反应发展和过敏症状。最近，维生素D（VitD）已被建议在减少过敏方面也具有有益作用。目的：本研究研究了GFA和VitD联合使用对预防过敏的免疫调节作用。方法：在乳清致敏之前和期间，对雌性C3H / HeOuJ小鼠饲喂对照或含GFA的普通（1,000 IU / kg）或补充（5,000 IU / kg）VitD含量的饮食2周（ n= 10-15）。每周一次，以PBS作为对照，以乳清作为牛乳过敏原，和/或将霍乱毒素作为佐剂对小鼠进行5次胃内敏化。在最后一次致敏后一周，对小鼠的耳廓和口服乳清进行了皮内攻击，随后测量了急性过敏性皮肤反应和休克症状。18小时后，收集末梢血，肠系膜淋巴结和脾脏。通过ELISA测量乳清特异性免疫球蛋白（Ig）E和IgG1水平。使用流式细胞仪研究了T细胞亚群和树突状细胞（DC）。结果：与标准VitD饮食相比，额外的VitD补充并未降低过敏症状。用补充VitD（GFA VitD ⁺）与喂VitD（VitD ⁺）组的CMA小鼠相比，乳清致敏小鼠的急性过敏性皮肤反应明显降低（p＃x3c; 0.05）。即使用GFA VitD ⁺饮食喂养的CMA小鼠与VitD ⁺组相比，CD103 ⁺ DC的百分比显着增加，但通过额外添加VitD不能改善GFA的作用（p＃x3c； 0.05）。缺乏VitD的小鼠表现出很高的严重休克率，许多小鼠达到了人道的终点。因此，这些组没有进一步分析。结论：在存在或不存在GFA的情况下，大剂量补充VitD不能抵抗CMA的发展。
Int Arch过敏免疫