A combination of alkaline thermal pretreatment followed by thermomechanical extrusion was studied as a novel sequential pretreatment process for an effective breakdown of the lignocellulosic structure of Eucalyptus grandis wood (EW). The first step was studied by analysing the influence of two factors: the NaOH-to-dry biomass ratio or NaOH loading (NaOH/DM) and the liquid-to-solid ratio (L/S). Optimization of these two parameters provided good results in terms of enzymatic hydrolysis at 5% (w w −1 ) solids loading, obtaining a total sugar concentration of 24.9 g L −1 and a total sugar production of 36.9 g 100 g −1 raw EW after pretreating the biomass at 20% NaOH/DM and L/S = 1/1. The second step of extrusion, when followed by a final washing step, provided a significant increase in glucose and xylose production when working at 10% NaOH/DM. For a soda loading of 20%, there was a clear improvement in sugars conversion yield after extrusion and washing: 71% for glucan conversion and 89% for xylan.

中文翻译：

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碱热预处理及热机械挤压工艺参数对桉木木材糖分释放影响的综合研究

研究了碱热预处理与热机械挤压相结合的方法，以此作为一种新的顺序预处理方法，可有效分解桉树木材（EW）的木质纤维素结构。通过分析两个因素的影响来研究第一步：NaOH与干生物质之比或NaOH装载量（NaOH / DM）和液对固比率（L / S）。在固含量为5％（ww -1）的条件下进行酶水解时，这两个参数的优化提供了良好的结果，总糖浓度为24.9 g L -1，总糖产量为36.9 g 100 g -1原始EW在20％NaOH / DM和L / S = 1/1的条件下预处理生物质。当在最后的洗涤步骤之后进行挤出的第二步，当在10％NaOH / DM下工作时，葡萄糖和木糖的产量显着增加。