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The transcriptional response of Arcobacter butzleri to cold shock.
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-08-18 , DOI: 10.1002/2211-5463.12959
Xiaochen Zhang 1 , Yulan Su 1 , Thomas Alter 1 , Greta Gölz 1
Affiliation  

Arcobacter (A.) butzleri is an emerging zoonotic pathogen associated with gastrointestinal diseases, such as abdominal cramps and diarrhea, and is widely detected in animals, showing a high prevalence in poultry and seafood. The survival and adaptation of A. butzleri to cold temperatures remains poorly studied, although it might be of interest for food safety considerations. To address this, growth patterns of eight A. butzleri isolates were determined at 8 °C for 28 days. A. butzleri isolates showed strain‐dependent behavior: six isolates were unculturable after day 18, one exhibited declining but detectable cell counts until day 28 and one grew to the stationary phase level. Out of 13 A. butzleri cold shock‐related genes homologous to Escherichia coli, 10 were up‐regulated in response to a temperature downshift to 8 °C, as demonstrated by reverse transcription‐quantitative PCR. Additionally, we compared these data with the cold‐shock response in E. coli. Overall, we provide a deeper insight into the environmental adaptation capacities of A. butzleri, which we find shares similarities with the E. coli cold‐shock response.

中文翻译:

Arcobacter butzleri 对冷休克的转录反应。

Arcobacter  ( A .)  butzleri是一种与胃肠道疾病相关的新兴人畜共患病病原体,如腹部绞痛和腹泻,在动物中广泛检测到,在家禽和海鲜中流行率很高。A. butzleri的生存和适应低温的研究仍然很少,尽管它可能对食品安全考虑感兴趣。为了解决这个问题,八种A. butzleri分离株的生长模式在 8 °C 下测定 28 天。A. butzleri分离株表现出菌株依赖性行为:6 株在第 18 天后无法培养,1 株在第 28 天前表现出下降但可检测到的细胞计数,1 株生长至稳定期水平。13个逆转录定量 PCR 表明,与大肠杆菌同源的A. butzleri冷休克相关基因10 在温度下降至 8 °C 时上调。此外,我们将这些数据与大肠杆菌的冷休克反应进行了比较。总体而言,我们对A. butzleri的环境适应能力提供了更深入的了解,我们发现它与大肠杆菌的冷休克反应有相似之处。
更新日期:2020-10-02
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