The present study demonstrates the efficacy of consortium inoculum formulated with two salt-tolerant, 1-Aminocyclopropane-1-carboxylate (ACC) deaminase producing, Aneurinibacillus aneurinilyticus strain AIOA1 (MH645748) and Paenibacillus sp. strain SG_AIOA2 (MH645749) on common bean plants grown under saline stress (100 mM) conditions. Initially, the common bean seeds were inoculated by coating with bacterial consortium for seedling performance under saline stress conditions. The consortium inoculated common bean seedlings has down-regulated the ethylene levels by ~61% and its associated growth inhibition through ACC deaminase activity. It has significantly enhanced seed germination percentage, root/shoot length, fresh weight and dry weight of root/shoot biomass, relative water content (RWC), chlorophyll and carotenoid content as well as osmoprotectants (proline, total soluble sugar) levels of salt exposed common bean plants. Furthermore, consortium application decreased the membrane permeability, electrolyte leakage as well as oxidative stress biomarkers (MDA and H₂O₂) content of common bean plants. The inoculants scavenged salt-induced reactive oxygen species (ROS) by enhancing the activities of antioxidative defensive enzymes (POD, SOD, CAT) and phenolics content. The strains have the potential to successfully colonize the root surface of plants confirmed by the colony-forming units (CFU) count method as well as bright field and scanning electron microscopy (SEM). The highest occurrence of ACC deaminase producing PGPR was found at root tips of fine roots. We conclude that our two strains are useful bio-inoculants to mitigate the negative impact of salt stress on common bean plants, and these should be field tested to verify their usefulness.

本研究证明了由两种耐盐，1-氨基环丙烷-1-羧酸盐（ACC）脱氨酶产生的解链菌，解旋神经耶氏杆菌AIOA1（MH645748）和Paenibacillus的功效sp。盐胁迫（100 mM）条件下生长的普通豆类植物上的SG_AIOA2菌株（MH645749）。最初，用盐渍菌包被接种普通豆种子，以在盐胁迫条件下提高幼苗的生长性能。该协会接种的普通豆苗已将乙烯含量下调了约61％，并通过ACC脱氨酶活性将其相关的生长抑制。它显着提高了种子暴露于盐中的种子的发芽率，根/茎长，根/茎生物量的鲜重和干重，相对水分含量（RWC），叶绿素和类胡萝卜素含量以及渗透保护剂（脯氨酸，总可溶性糖）的水平。普通豆类植物。此外，财团的应用降低了膜的渗透性，电解质的渗漏以及氧化应激生物标志物（MDA和H₂ O ₂）含量的普通豆类植物。孕育剂通过增强抗氧化防御酶（POD，SOD，CAT）和酚类成分的活性来清除盐诱导的活性氧（ROS）。通过菌落形成单位（CFU）计数法以及明场和扫描电子显微镜（SEM）证实，该菌株具有成功定植植物根部表面的潜力。在细根的根尖发现ACC脱氨酶产生PGPR的发生率最高。我们得出的结论是，我们的两种菌株是有用的生物杀菌剂，可减轻盐胁迫对普通豆类植物的负面影响，因此应对它们进行现场测试，以验证其有效性。