Directed stabilization of globular proteins via substitution of a minimal number of amino acid residues is one of the most complicated experimental tasks. In this work, we have successfully used algorithms for the evaluation of intrinsic disorder predisposition (such as PONDR® FIT and IsUnstruct) as tools for searching for the weakened regions in structured globular proteins. We have shown that the weakened regions found by these programs as regions with highest levels of predicted intrinsic disorder predisposition are a suitable target for introduction of stabilizing mutations.

中文翻译：

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基于内在疾病的稳定球状蛋白设计。

通过取代最少数量的氨基酸残基来直接稳定球状蛋白是最复杂的实验任务之一。在这项工作中，我们成功地使用了算法来评估固有疾病的易患性（例如PONDR®FIT和IsUnstruct），作为在结构化球状蛋白中寻找弱化区域的工具。我们已经表明，这些程序所发现的弱化区域是具有最高水平的固有疾病易感性的区域，是引入稳定突变的合适目标。