Abstract Tremendous economic losses in the citrus industry are caused by infestations of Penicillium digitatum. Previous research has shown that the terpene myrcene in citrus essential oils could stimulate the spore germination and mycelial growth of P. digitatum. However, the underlying mechanism is poorly known. Here, how myrcene induces the spore germination of P. digitatum was elucidated. The addition of myrcene significantly promoted the spore germination of P. digitatum and green mold in citrus fruit. As the incubation time increased, the intracellular and extracellular myrcene contents both significantly decreased in myrcene-treated samples, and no downstream products were detected through a Gas Chromatography-Mass Spectrometer. Metabolomics analysis revealed that the contents of metabolites involved in central carbon metabolism, such as glycolysis, pentose phosphate pathway, and citrate cycle, were significantly affected after the addition of myrcene. The adenosine triphosphate content and the intracellular energy charge were also drastically increased. Overall, our results suggest that the ability of myrcene to stimulate the spore germination of P. digitatum might be mediated by the upregulation of central carbon and energy metabolism.

中文翻译：

---

代谢组学分析表明，月桂烯通过上调中心碳和能量代谢来刺激指状青霉孢子萌发

摘要 指状青霉的侵染给柑橘产业造成了巨大的经济损失。先前的研究表明，柑橘精油中的萜烯月桂烯可以刺激 P. digitatum 的孢子萌发和菌丝生长。然而，其潜在机制知之甚少。在这里，阐明了月桂烯如何诱导 P. digitatum 的孢子萌发。月桂烯的添加显着促进了柑橘类果实中P. digitatum和绿霉的孢子萌发。随着孵育时间的增加，月桂烯处理样品的细胞内和细胞外月桂烯含量均显着降低，并且通过气相色谱-质谱仪未检测到下游产物。代谢组学分析表明，参与中心碳代谢的代谢物含量，添加月桂烯后，糖酵解、磷酸戊糖途径和柠檬酸循环等功能受到显着影响。三磷酸腺苷含量和细胞内能量电荷也急剧增加。总的来说，我们的结果表明月桂烯刺激 P. digitatum 孢子萌发的能力可能是由中央碳和能量代谢的上调介导的。