Allylglycine, a conventional amino acid derivative, possesses typical zwitterionic and hydrophilic functionalities deriving from the carboxyl and amino groups in its structure. A novel monolithic column poly(allylglycine-co-1, 3, 5-triacryloylhexahydro-1, 3, 5-triazine) (AGly-co-TAT) with powerful hydrophilic selectivity and obvious zwitterionic feature was synthesized successfully with the monomer allyglycine and the cross-linker 1, 3, 5-triacryloylhexahydro-1, 3, 5-triazine through in-situ copolymerization for capillary electrochromatography. The obtained monolithic column has good permeability. Due to the zwitterionic functional groups of allylglycine, the poly(AGly-co-TAT) monolithic column can generate a cathodic and anodic electroosmotic flow (EOF) by changing the mobile phase pH, which is beneficial to expand its application range. The separations of different series of polar analytes, thioureas, xanthines, phenols, peptides and acidic compounds are achieved on this hydrophilic monolithic column due to the powerful hydrophilic, electrostatic and hydrogen bond interactions. Using this monolithic column, hydrophilic separations are achieved even at a lower level of 50% organic solvent. The separation efficiency up to 1.41 × 10⁵ N m⁻¹ and 1.19 × 10⁵ N m⁻¹ is achieved for the separation of theophylline and phenol, respectively. For a real sample, cytochrome C digestion, the monolithic column shows good separation performance, which offers the potential application of the monolithic column on proteomics study.

烯丙基甘氨酸，一种常规的氨基酸衍生物，具有典型的两性离子和亲水性官能团，其衍生自其结构中的羧基和氨基。一种新型的整体柱聚（allylglycine-共-1，3，5-三丙烯-1，3，5 -三嗪）（AGly-共-TAT）具有强大的亲水性的选择性和明显两性离子功能是与单体allyglycine和成功地合成交联剂1，3，5-三丙烯酰基六氢-1，3，5-三嗪通过原位共聚进行毛细管电色谱分析。所得整体柱具有良好的渗透性。由于烯丙基甘氨酸的两性离子官能团，聚（AGly- co-TAT）整体柱可以通过改变流动相的pH值来产生阴极和阳极电渗流（EOF），这有利于扩大其应用范围。由于强大的亲水，静电和氢键相互作用，在该亲水整体柱上实现了不同系列的极性分析物，硫脲，黄嘌呤，酚，肽和酸性化合物的分离。使用该整体式色谱柱，即使在较低含量的50％有机溶剂下也可以实现亲水性分离。分离效率高达1.41×10 ⁵ N m ^-1和1.19×10 ⁵ N m ^-1分别实现了茶碱和苯酚的分离。对于真实的样品（细胞色素C消化），整体柱表现出良好的分离性能，这为整体柱在蛋白质组学研究中的潜在应用提供了可能。