In ovo administration of a live-attenuated F-strain Mycoplasma gallisepticum vaccine (FMG) has proven to be a promising alternative vaccination route for layer chickens to combat M. gallisepticum (MG) infections. Its practical application within current in ovo vaccination procedures, employing a disinfection step after each injection, has been uncertain. The objective of this experiment was to determine the ability of FMG to survive the in ovo disinfection step to elicit a posthatch immune response. In 2 trials, layer eggs were in ovo-vaccinated with or without the disinfection step between injections. Utilizing an Embrex Inovoject M machine, treatments included noninjected eggs, diluent-injected eggs with or without a disinfection step, and eggs vaccinated with an FMG vaccine with or without a disinfection step. Ninety hatched chicks per treatment in both trials were raised to 6 wk of age and swabbed in the choanal cleft for FMG detection via PCR and were bled for IgM (serum plate agglutination) and IgG (ELISA) testing for detection of antibody production against MG. The percentage of birds in which FMG was detected at 6 wk of age were similar among both FMG vaccination treatments in both trials (overall 89.4% without and 91.6% with disinfectant step). Birds vaccinated with FMG also developed antibody responses regardless of employment of the disinfection step. These results indicate that FMG survives the disinfection step employed by commercial machines and can be readily applied to current commercial procedures of in ovo vaccination with no detriment to vaccine efficacy.

卵内减毒的F株鸡毒支原体疫苗（FMG）的卵内给药已被证明是一种有前景的替代疫苗，可用于蛋鸡对抗鸡毒支原体（MG）感染。在每次注射后采用消毒步骤的卵内疫苗接种程序中，其实际应用尚不确定。该实验的目的是确定FMG在卵内消毒步骤中存活以引发孵化后免疫反应的能力。在2个试验中，在注射之间有或没有消毒步骤的情况下对卵中的卵进行了疫苗接种。使用Embrex Inovoject M机器，处理方法包括未注射的卵，有或没有消毒步骤的注射有稀释剂的卵，以及有或没有消毒步骤的用FMG疫苗接种的卵。在两个试验中，每个处理中有90只孵化的雏鸡被提高到6周龄，并在the沟中擦拭以通过PCR检测FMG，并放血进行IgM（血清平板凝集）和IgG（ELISA）检测以检测针对MG的抗体产生。在两项试验中，两种FMG疫苗接种方法中，在6周龄时均检测到FMG的禽鸟的百分比相似（不使用消毒剂步骤的总比例为89.4％，使用消毒步骤的比例为91.6％）。无论采用何种消毒步骤，接种FMG疫苗的禽鸟也会产生抗体反应。这些结果表明FMG在商业机器采用的消毒步骤中幸存下来，并且可以容易地应用于当前的卵内疫苗商业程序，而不会损害疫苗效力。在两项试验中，两种FMG疫苗接种方法中，在6周龄时均检测到FMG的禽鸟的百分比相似（不使用消毒剂步骤的总比例为89.4％，使用消毒步骤的比例为91.6％）。无论采用何种消毒步骤，接种FMG疫苗的禽鸟也会产生抗体反应。这些结果表明FMG在商业机器采用的消毒步骤中幸存下来，并且可以容易地应用于当前的卵内疫苗商业程序，而不会损害疫苗效力。在两项试验中，两种FMG疫苗接种方法中，在6周龄时均检测到FMG的禽鸟的百分比相似（不使用消毒剂步骤的总比例为89.4％，使用消毒步骤的比例为91.6％）。无论采用何种消毒步骤，接种FMG疫苗的禽鸟也会产生抗体反应。这些结果表明FMG在商业机器采用的消毒步骤中幸存下来，并且可以容易地应用于当前的卵内疫苗商业程序，而不会损害疫苗效力。