The cell lines derived from the fall armyworm (FAW), Spodoptera frugiperda, have been widely used for production of recombinant proteins for applications in both basic research and applications in medicine and agriculture. Promoters from the nucleopolyhedrovirus (NPV) are commonly used in these expression systems. These promoters have some limitations, which may be overcome by using promoters of genes from S. frugiperda. However, information on these promoters is not available. We identified several highly expressed genes from the transcriptomes of S. frugiperda midgut, fat body, epidermis, ovarian cell line (Sf9), and a midgut cell line (Sf17). The activity of potential promoters of 21 highly expressed genes was evaluated in Sf9 and Sf17 cells. Two of these promoters, SfHSC70-P1780 and SfPub-P2009, showed higher activity than commonly used hr5/ie1 (baculovirus enhancer element, hr5 and immediate early gene 1, ie1) promoter. Interestingly, the activity of these two promoters increased after adding hr5 enhancer element. The hr5/SfPub-P2009 promoter performance was evaluated by expressing an exogenous P450 protein in Sf9 cells using a plasmid-based expression system. The activity of this promoter was also evaluated in the FAW by expressing green fluorescence protein using the baculovirus expression system. In both cases, the hr5/SfPub-P2009 promoter performed better than the commonly used hr5/ie1 promoter. These strong endogenous promoters will be useful for studies in S. frugiperda and other lepidopteran insects for multiple applications, including protein expression, genome editing, and transgenic insects.

源自秋天粘虫（FAW）Spodoptera frugiperda的细胞系已广泛用于重组蛋白的生产，可用于基础研究以及医学和农业应用。在这些表达系统中通常使用核多角体病毒（NPV）的启动子。这些启动子具有某些局限性，可以通过使用弗氏链球菌的基因启动子来克服。但是，没有有关这些启动子的信息。我们从S. frugiperda中肠，脂肪体，表皮，卵巢细胞系（Sf9）和中肠细胞系（Sf17）的转录组中鉴定了几个高度表达的基因。在Sf9和Sf17细胞中评估了21种高表达基因的潜在启动子的活性。其中两个启动子SfHSC70 -P1780和SfPub -P2009显示出比常用的hr5 / ie1（杆状病毒增强子hr5和立即早期基因1，即ie1）启动子更高的活性。有趣的是，加入hr5增强子后，这两个启动子的活性增加了。通过使用基于质粒的表达系统在Sf9细胞中表达外源P450蛋白来评估hr5 / SfPub -P2009启动子的性能。还通过使用杆状病毒表达系统表达绿色荧光蛋白在一汽中评估了该启动子的活性。在两种情况下，hr5 / SfPub-P2009启动子的表现优于常用的hr5 / ie1启动子。这些强大的内源性启动子可用于在S. frugiperda和其他鳞翅目昆虫中进行多种应用的研究，包括蛋白质表达，基因组编辑和转基因昆虫。