Abstract We developed and synthesized dimeric pyrene with pyridine unit based metal biding fluorescent probe to screening sequence of metal ions. The analyzed pattern showed a clear distinction of responsive and non-responsive metal ions. From the successful experiment, we found that the synthesized probe is selectively responsive to Hg22+ and Pb2+ ions through both visually and optically. This is the first fluorescent chemosensor for Hg22+ based on pyrene. The quantitative aspects of the detection process has been calculated by absorption, emission, lifetime decay and quantum yield study. Eventually, using confocal laser scanning microscopy experiments we attempted to testify the drastic changes in bacterial intracellular cells by inducing fluorescence emission by the combination of the probe with Hg22+ and similarly probe with Pb2+.

中文翻译：

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带有完美杂原子腔光学传感器的扭曲芘用于 Hg22+ 和 Pb2+

摘要 我们开发合成了基于吡啶单元的二聚芘金属结合荧光探针来筛选金属离子序列。分析的模式显示了响应和非响应金属离子的明显区别。从成功的实验中，我们发现合成的探针通过视觉和光学选择性地响应 Hg22+ 和 Pb2+ 离子。这是第一个基于芘的 Hg22+ 荧光化学传感器。通过吸收、发射、寿命衰减和量子产率研究计算了检测过程的定量方面。最后，使用共聚焦激光扫描显微镜实验，我们试图通过探针与 Hg22+ 和类似探针与 Pb2+ 的组合诱导荧光发射来证明细菌细胞内细胞的剧烈变化。