Deglycosylation has previously been suggested to modulate the biological activities of various phytochemicals. The current study was designed to evaluate the effects of deglycosylation on antioxidant potential of crude methanolic extracts (CMEs) of four medicinal plants: Adhatoda vasica, Skimmia laureola, Olea ferruginea, and Cymbopogon citratus. The CMEs were prepared in 80% methanol and their antioxidant activity was measured using DPPH (diphenyl picryl hydrazyl) photometric assay. The resultant IC50 values were 82 μg/mL for O. ferruginea, 127 μg/mL for S. laureola, 169 μg/mL for C. citrates, 170 μg/mL for A. vasica, and 3.1 μg/mL for ascorbic acid, which was used as a standard. Glycosidase enzyme was extracted from Medicago sativa (alfalfa) seeds and assayed chromogenically using X-gal as its substrate. The glycosidase enzyme successfully deglycosylated CMEs of all the four plants. These degylosylated extracts were also analyzed for their antioxidant activities. New IC50 values were 52 μg/mL for O. ferruginea, 71 μg/mL for S. laureola, 103 μg/mL for A. vasica, and 111 μg/mL for C. citratus. These observations show a significant increase in the antioxidant potential of all four CMEs as a result of their deglycosylation. All results were analyzed statistically and significant difference (p = 0) was established between CMEs and deglycosylated CMEs of all the four selected plants tested separately.

中文翻译：

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去糖基化对选定植物提取物的抗氧化潜力的作用

先前已建议去糖基化调节各种植物化学物质的生物活性。目前的研究旨在评估去糖基化对四种药用植物粗甲醇提取物 (CME) 的抗氧化潜力的影响：Adhatoda vasica、Skimmia laureola、Olea ferruginea 和 Cymbopogon citratus。CME 在 80% 甲醇中制备，并使用 DPPH（二苯基苦基肼）光度测定法测量其抗氧化活性。所得的 IC50 值为 O. ferruginea 82 μg/mL、S. laureola 127 μg/mL、C. citrates 169 μg/mL、A. vasica 170 μg/mL 和抗坏血酸 3.1 μg/mL，这被用作标准。从苜蓿（苜蓿）种子中提取糖苷酶，并使用 X-gal 作为其底物进行显色分析。糖苷酶成功地使所有四种植物的 CME 去糖基化。还分析了这些去糖基化提取物的抗氧化活性。新的 IC50 值为 O. ferruginea 52 μg/mL、S. laureola 71 μg/mL、A. vasica 103 μg/mL 和 C. citratus 111 μg/mL。这些观察结果表明，由于去糖基化，所有四种 CME 的抗氧化潜力都显着增加。对所有结果进行统计学分析，并在分别测试的所有四种选定植物的 CME 和去糖基化 CME 之间建立了显着差异 (p = 0)。这些观察结果表明，由于去糖基化，所有四种 CME 的抗氧化潜力都显着增加。对所有结果进行统计学分析，并在分别测试的所有四种选定植物的 CME 和去糖基化 CME 之间建立了显着差异 (p = 0)。这些观察结果表明，由于去糖基化，所有四种 CME 的抗氧化潜力都显着增加。对所有结果进行统计学分析，并在分别测试的所有四种选定植物的 CME 和去糖基化 CME 之间建立了显着差异 (p = 0)。