Silver nanoparticles (AgNPs) induced specific cell toxicity, and they are used as a tool for the study of several pathologies such as cancer. This work aimed to elucidate the toxic effect of < 10-nm silver nanoparticles (AgNPs) and zinc chloride (ZnCl₂) in different administration orders on C6 rat glioma cells, as a biological model of study. C6 rat glioma cells were exposed to increasing concentrations of AgNPs (10–100 μg/mL) in the presence or absence of ZnCl₂ (10–50 μg/mL) for 24 h. AgNPs or ZnCl₂ as separate treatments decreased C6 rat glioma cell viability by 21% and 13%, respectively, versus the control, using the MTT assay. The administration of AgNPs (50 μg/mL) in the presence of ZnCl₂ (10–50 μg/mL) was performed under two conditions: as pretreatment and as concomitant administration; both of them showed a significant decrease in the cell viability, around 30% and 90%, respectively. It was the concomitant treatment, which exerted the most significant effect on the viability decrease. We also observed that 24-h exposure to AgNPs increased cell populations (40%) in stages G0/G1 of the cell cycle, and decreased the number of cells (60%) in stages G2/M. However, in the concomitant treatment, as well as during induced cell death, the ZnCl₂ pretreatment and concomitant treatment modified the cycle, increasing the S phase by 10%, suggesting that zinc (Zn) could be an essential regulator of the C6 rat glioma cell damage induced by AgNPs. This study will allow us to understand the mechanisms of cellular response to AgNPs, for the eventual study of these particles as a potential agent against cancer, such as glioblastoma multiforme.

银纳米颗粒（AgNPs）诱导特定的细胞毒性，它们被用作研究多种病理学例如癌症的工具。这项工作旨在阐明<10 nm纳米银纳米颗粒（AgNPs）和氯化锌（ZnCl ₂）在不同给药顺序对C6大鼠神经胶质瘤细胞的毒性作用，作为生物学研究模型。在存在或不存在ZnCl ₂（10-50μg/ mL）的情况下，将C6大鼠神经胶质瘤细胞暴露于浓度不断升高的AgNP（10-100μg/ mL）中，持续24小时。使用MTT分析法，与对照组相比，AgNPs或ZnCl ₂分别降低C6大鼠神经胶质瘤细胞的存活率21％和13％。在ZnCl ₂存在下施用AgNPs（50μg/ mL）（10–50μg/ mL）在两个条件下进行：作为预处理和同时给药；它们都显示出细胞活力的显着降低，分别约为30％和90％。伴随的治疗对存活率降低具有最显着的影响。我们还观察到，在细胞周期的G0 / G1阶段，暴露于AgNPs 24小时可增加细胞数量（40％），而在G2 / M阶段则减少细胞数量（60％）。然而，在伴随治疗以及诱导细胞死亡期间，ZnCl ₂预处理和伴随治疗改变了周期，使S期增加了10％，这表明锌（Zn）可能是AgNPs诱导的C6大鼠神经胶质瘤细胞损伤的重要调节剂。这项研究将使我们了解细胞对AgNPs的反应机制，以便最终研究这些颗粒作为潜在的抗癌剂，例如多形胶质母细胞瘤。