Three novel viruses were detected from Japanese persimmon ‘Reigyoku’ (Diospyros kaki), which grows poorly when top-grafted onto some interstocks. The genomic characterization and the phylogenetic analysis of these viruses indicated that they are likely new members of the genera Ampelovirus, Polerovirus, and Waikavirus, tentatively named Persimmon ampelovirus (PAmpV), Persimmon polerovirus (PPolV), and Persimmon waikavirus (PWaiV). The alignment of two PAmpV variants with Plum bark necrosis stem pitting–associated virus isolates had 72.5%–79.0% amino acid sequence identities with RNA-dependent RNA polymerase, 71.6%–79.2% with a heat shock protein 70 homolog, and 66.2%–69.8% with coat protein, respectively. Two PPolV variants showed the highest amino acid sequence identities (46.6%) with the P1–P2 fusion protein of Brassica yellows virus. PWaiV had the highest amino acid sequence identity (41.9%) with the conserved domain between proteinase and polymerase of Rice tungro spherical virus. Reverse-transcription and polymerase chain reaction detected Persimmon virus A, Apple crinkle fruit viroid, and Citrus viroid VI from the mother clone with good growth, and PAmpV, PPolV, and Persimmon latent viroid (PLVd) additionally from 20 trees with poor growth. PAmpV, PPolV, and PLVd were detected from four, 10, and 10 of 14 trees with good growth, respectively, and the grafting of budwood harboring these three pathogens caused significant stunt in mother clones.

从日本柿子“ Reigyoku”（Diospyros kaki）中检测到三种新型病毒，当将其顶部嫁接到某些中间种群时生长较差。这些病毒的基因组表征和系统发育分析表明，它们很可能是两性病毒，杆状病毒和怀卡病毒属的新成员，暂定名为柿子ampelvirus（PAmpV），柿子杆状病毒（PPolV）和柿子waikavirus（PWaiV）。两个PAmpV变体与梅树皮坏死茎点蚀相关病毒的比对分离株与依赖RNA的RNA聚合酶的氨基酸序列同一性为72.5％–79.0％，与热激蛋白70同源物的分离株分别为71.6％–79.2％，与外壳蛋白为66.2％–69.8％。两个PPolV变异体与芸苔黄病毒的P1-P2融合蛋白显示出最高的氨基酸序列同一性（46.6％）。PWaiV与水稻通果球形病毒的蛋白酶和聚合酶之间的保守结构域具有最高的氨基酸序列同一性（41.9％）。逆转录和聚合酶链反应检测到柿病毒A，苹果皱纹水果类病毒和柑橘类病毒VI来自生长良好的母本克隆，以及来自生长不良的20棵树的PAmpV，PPolV和柿子潜在类病毒（PLVd）。在14棵生长良好的树木中分别检测到4、10和10棵树中的PAmpV，PPolV和PLVd，带有这三种病原体的芽木的嫁接在母克隆中造成了显着的发育不良。