The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating that Cryptococcus sp. MR22 and Halomonas sp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations. The fit model to represent the biodegradation kinetics of both PAHs was first-order rate equation The extract prepared from cells supplemented with three different substrates exhibited some enzymes such as hydroxylase, dioxygenase, laccase and peroxidase. The results suggest that both strains had an impressive ability in the degradation of aromatic and aliphatic hydrocarbon but also could tolerate in the extreme salinity condition.

本研究旨在确定隐球菌对三环多环芳烃菲和蒽的降解和转化。MR22 和Halomonas sp。BR04 在嗜盐条件下。隐球菌的生长进展。MR22 和Halomonas sp。通过菌落形成单位（CFU）技术监测蒽和菲上的BR04。细菌的生长保持在所有测试碳氢化合物的最大浓度为 200 毫克/升，表明隐球菌属。MR22 和盐单胞菌sp. BR04 在去除低浓度多环芳烃污染介质方面表现出色。代表两种多环芳烃生物降解动力学的拟合模型为一级速率方程。从补充了三种不同底物的细胞中制备的提取物表现出一些酶，如羟化酶、双加氧酶、漆酶和过氧化物酶。结果表明，这两种菌株在降解芳香烃和脂肪烃方面都具有令人印象深刻的能力，而且在极端盐度条件下也能耐受。