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Peripheral Blood Inflammatory-Immune Cells as a Predictor of Infertility in Women with Polycystic Ovary Syndrome.
Journal of Inflammation Research ( IF 4.2 ) Pub Date : 2020-08-18 , DOI: 10.2147/jir.s260770 ShuQiong He 1 , XiaoDan Mao 2, 3 , HuiFang Lei 2, 3 , BinHua Dong 2, 3 , DanHua Guo 1 , BeiHong Zheng 4 , PengMing Sun 2, 3
Journal of Inflammation Research ( IF 4.2 ) Pub Date : 2020-08-18 , DOI: 10.2147/jir.s260770 ShuQiong He 1 , XiaoDan Mao 2, 3 , HuiFang Lei 2, 3 , BinHua Dong 2, 3 , DanHua Guo 1 , BeiHong Zheng 4 , PengMing Sun 2, 3
Affiliation
Purpose: This study aimed to investigate the inflammatory-immune cells in the peripheral blood of women with polycystic ovary syndrome (PCOS) and assessed the potential correlation between inflammatory-immune cells and infertility in PCOS women.
Materials and Methods: In this case–control study, the profiles of lymphocyte subsets were analyzed by flow cytometry. White blood cells (WBC), neutrophils (Neu), lymphocytes, Ferriman–Gallwey (F–G) score, testosterone, prolactin, follicle-stimulating hormone, luteinizing hormone, fasting blood glucose, and fasting plasma insulin were measured, together with body mass index. Association between inflammatory-immune cells and PCOS was evaluated. Moreover, inflammatory-immune cells of the PCOS women with infertility were evaluated, and the relative operating characteristic (ROC) curve and cutoff values were calculated.
Results: The number of WBC, Neu, and lymphocytes was higher in PCOS women than controls (P< 0.05). The percentages of total T lymphocytes, CD4+T, and NK were significantly increased in the PCOS group (P< 0.001). The CD4/CD8 ratio was obviously elevated for increasing CD4+T (P< 0.05). Consequently, T%, CD4+T%, and NK% were found to be the independent risk factors of PCOS by ROC curve and multivariate logistic regression analysis. Furthermore, only NK% was significantly higher in PCOS women with infertility than those who had PCOS without infertility (P< 0.001). To diagnose infertility in PCOS, the cutoff value of NK% was calculated as 16.43%.
Conclusion: These findings suggest that the pathogenesis of PCOS is related to immune cells including T, CD4+T, and NK cells. NK cells are likely to be a potential predictive factor for PCOS women with infertility.
Keywords: lymphocyte subsets, inflammatory cells, natural killer cells, immunocompetent cells, pathogenesis
中文翻译：
外周血炎性免疫细胞作为多囊卵巢综合征女性不孕症的预测因子。
目的：本研究旨在调查多囊卵巢综合征 (PCOS) 女性外周血中的炎症免疫细胞，并评估炎症免疫细胞与 PCOS 女性不孕症之间的潜在相关性。
材料和方法：在本病例对照研究中，通过流式细胞术分析淋巴细胞亚群的分布。测量白细胞（WBC）、中性粒细胞（Neu）、淋巴细胞、Ferriman-Gallwey（F-G）评分、睾酮、催乳素、促卵泡激素、促黄体激素、空腹血糖和空腹血浆胰岛素，以及身体质量指数。评估了炎症免疫细胞与 PCOS 之间的关联。此外，还评估了 PCOS 女性不孕症的炎症免疫细胞，并计算了相对操作特征 (ROC) 曲线和截止值。
结果：PCOS 女性的 WBC、Neu 和淋巴细胞数量高于对照组（P<0.05）。PCOS组总T淋巴细胞、CD4+T和NK的百分比显着增加（P<0.001）。CD4/CD8比值随着CD4+T的升高而明显升高（P<0.05）。因此，通过ROC曲线和多因素logistic回归分析发现T%、CD4+T%和NK%是PCOS的独立危险因素。此外，仅 PCOS 不育妇女的 NK% 显着高于未生育 PCOS 的妇女（P<0.001）。为了诊断 PCOS 的不孕症，NK% 的临界值计算为 16.43%。
结论：这些发现表明PCOS的发病机制与包括T细胞、CD4+T细胞和NK细胞在内的免疫细胞有关。NK 细胞可能是 PCOS 女性不孕症的潜在预测因素。
关键词：淋巴细胞亚群，炎症细胞，自然杀伤细胞，免疫活性细胞，发病机制
更新日期：2020-08-18
Materials and Methods: In this case–control study, the profiles of lymphocyte subsets were analyzed by flow cytometry. White blood cells (WBC), neutrophils (Neu), lymphocytes, Ferriman–Gallwey (F–G) score, testosterone, prolactin, follicle-stimulating hormone, luteinizing hormone, fasting blood glucose, and fasting plasma insulin were measured, together with body mass index. Association between inflammatory-immune cells and PCOS was evaluated. Moreover, inflammatory-immune cells of the PCOS women with infertility were evaluated, and the relative operating characteristic (ROC) curve and cutoff values were calculated.
Results: The number of WBC, Neu, and lymphocytes was higher in PCOS women than controls (P< 0.05). The percentages of total T lymphocytes, CD4+T, and NK were significantly increased in the PCOS group (P< 0.001). The CD4/CD8 ratio was obviously elevated for increasing CD4+T (P< 0.05). Consequently, T%, CD4+T%, and NK% were found to be the independent risk factors of PCOS by ROC curve and multivariate logistic regression analysis. Furthermore, only NK% was significantly higher in PCOS women with infertility than those who had PCOS without infertility (P< 0.001). To diagnose infertility in PCOS, the cutoff value of NK% was calculated as 16.43%.
Conclusion: These findings suggest that the pathogenesis of PCOS is related to immune cells including T, CD4+T, and NK cells. NK cells are likely to be a potential predictive factor for PCOS women with infertility.
Keywords: lymphocyte subsets, inflammatory cells, natural killer cells, immunocompetent cells, pathogenesis
中文翻译：
外周血炎性免疫细胞作为多囊卵巢综合征女性不孕症的预测因子。
目的：本研究旨在调查多囊卵巢综合征 (PCOS) 女性外周血中的炎症免疫细胞，并评估炎症免疫细胞与 PCOS 女性不孕症之间的潜在相关性。
材料和方法：在本病例对照研究中，通过流式细胞术分析淋巴细胞亚群的分布。测量白细胞（WBC）、中性粒细胞（Neu）、淋巴细胞、Ferriman-Gallwey（F-G）评分、睾酮、催乳素、促卵泡激素、促黄体激素、空腹血糖和空腹血浆胰岛素，以及身体质量指数。评估了炎症免疫细胞与 PCOS 之间的关联。此外，还评估了 PCOS 女性不孕症的炎症免疫细胞，并计算了相对操作特征 (ROC) 曲线和截止值。
结果：PCOS 女性的 WBC、Neu 和淋巴细胞数量高于对照组（P<0.05）。PCOS组总T淋巴细胞、CD4+T和NK的百分比显着增加（P<0.001）。CD4/CD8比值随着CD4+T的升高而明显升高（P<0.05）。因此，通过ROC曲线和多因素logistic回归分析发现T%、CD4+T%和NK%是PCOS的独立危险因素。此外，仅 PCOS 不育妇女的 NK% 显着高于未生育 PCOS 的妇女（P<0.001）。为了诊断 PCOS 的不孕症，NK% 的临界值计算为 16.43%。
结论：这些发现表明PCOS的发病机制与包括T细胞、CD4+T细胞和NK细胞在内的免疫细胞有关。NK 细胞可能是 PCOS 女性不孕症的潜在预测因素。
关键词：淋巴细胞亚群，炎症细胞，自然杀伤细胞，免疫活性细胞，发病机制
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