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Three-Dimensional DNA Nanomachine Combined with Toehold-Mediated Strand Displacement Reaction for Sensitive Electrochemical Detection of MiRNA.
Langmuir ( IF 3.7 ) Pub Date : 2020-08-17 , DOI: 10.1021/acs.langmuir.0c01415 Hanwen Lu 1 , Tang Hailin 2 , Xinyao Yi 1 , Jianxiu Wang 1
Langmuir ( IF 3.7 ) Pub Date : 2020-08-17 , DOI: 10.1021/acs.langmuir.0c01415 Hanwen Lu 1 , Tang Hailin 2 , Xinyao Yi 1 , Jianxiu Wang 1
Affiliation
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MicroRNA (miRNA) serves as an ideal biomarker for diagnosis, prognosis, and therapy of various human cancers. The rationally designed three-dimensional (3D) DNA nanomachine was constructed on the matrixes of magnetic beads, and the high density of gold nanoparticles (AuNPs) on each magnetic bead and further enlargement of the AuNPs lead to the anchoring of numerous DNA walkers and signal probes on the AuNPs. With the combination of toehold-mediated strand displacement reaction (SDR), amplified electrochemical detection of miRNA is performed. The existence of miRNA triggers the toehold-mediated SDR and the released DNA walker probe is hybridized with the ferrocene (Fc)-tagged signal probe. The cleavage of the duplex by the nicking endonuclease detaches the signal probe from the magnetic nanocomposites. The oxidation current of Fc moieties was found to be inversely proportional to the concentrations of miRNA-182 between 1.0 fM and 2 pM. The assay is highly selective for discrimination of miRNAs with similar sequences. The feasibility of the method for sensitive detection of the expression levels of miRNA-182 from serum samples of glioma patients at different stages was demonstrated. The sensing protocol holds great promise for early diagnosis and prognosis of the cancer cases with abnormal miRNA expression.
中文翻译：
三维DNA纳米机结合脚趾介导的链置换反应对MiRNA进行灵敏的电化学检测。
MicroRNA（miRNA）可作为诊断，预后和治疗各种人类癌症的理想生物标志物。在磁珠的基质上构建了合理设计的三维（3D）DNA纳米机器，每个磁珠上的高密度金纳米颗粒（AuNPs）以及AuNPs的进一步扩大导致了众多DNA Walker和信号的锚定AuNPs上的探针。结合脚趾介导的链置换反应（SDR），对miRNA进行放大的电化学检测。miRNA的存在触发了脚趾介导的SDR，释放的DNA Walker探针与二茂铁（Fc）标记的信号探针杂交。切口核酸内切酶对双链体的切割使信号探针与磁性纳米复合材料分离。发现Fc部分的氧化电流与1.0fM至2pM之间的miRNA-182的浓度成反比。该测定法对区分具有相似序列的miRNA具有高度选择性。证明了该方法灵敏检测神经胶质瘤患者不同阶段血清样品中miRNA-182表达水平的可行性。传感方案为miRNA表达异常的癌症病例的早期诊断和预后提供了广阔的前景。
更新日期：2020-09-15
中文翻译：
三维DNA纳米机结合脚趾介导的链置换反应对MiRNA进行灵敏的电化学检测。
MicroRNA（miRNA）可作为诊断，预后和治疗各种人类癌症的理想生物标志物。在磁珠的基质上构建了合理设计的三维（3D）DNA纳米机器，每个磁珠上的高密度金纳米颗粒（AuNPs）以及AuNPs的进一步扩大导致了众多DNA Walker和信号的锚定AuNPs上的探针。结合脚趾介导的链置换反应（SDR），对miRNA进行放大的电化学检测。miRNA的存在触发了脚趾介导的SDR，释放的DNA Walker探针与二茂铁（Fc）标记的信号探针杂交。切口核酸内切酶对双链体的切割使信号探针与磁性纳米复合材料分离。发现Fc部分的氧化电流与1.0fM至2pM之间的miRNA-182的浓度成反比。该测定法对区分具有相似序列的miRNA具有高度选择性。证明了该方法灵敏检测神经胶质瘤患者不同阶段血清样品中miRNA-182表达水平的可行性。传感方案为miRNA表达异常的癌症病例的早期诊断和预后提供了广阔的前景。
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