Sustainable Solvothermal Conversion of Waste Biomass to Functional Carbon Material: Extending Its Utility as a Biocompatible Cosolvent for Lysozyme,ACS Biomaterials Science & Engineering

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Sustainable Solvothermal Conversion of Waste Biomass to Functional Carbon Material: Extending Its Utility as a Biocompatible Cosolvent for Lysozyme
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-08-17 , DOI: 10.1021/acsbiomaterials.0c00461
Karan Chaudhary ₁ , Kavya Bhakuni ₁ , Navin Kumar Mogha ₁ , Pannuru Venkatesu ₁ , Dhanraj T. Masram ₁

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Functional carbon material synthesis from waste biomass by a sustainable method is of prime importance and has wide variety of applications. Herein, functional carbon materials with structural variability are synthesized using a well-known solvothermal method. The leftover pulp waste biomass (PB) of citrus limetta is converted to functional carbon by treatment with a mixture of choline bitartrate (ChBt) and FeCl₃ (1:2 mol ratio) as a solvent. The biomass to solvent ratio is varied as 1:1, 0.8:1, and 0.4:1 during solvothermal treatment to obtain PB-1, PB-2, and PB-3 as functional carbon materials, respectively. On characterization, PB carbon materials were found to be rich in oxygen-containing functional groups possessing different morphologies. Furthermore, results suggested the role of solvent as a soft template and catalyst during the synthesis of carbon materials. The feasibility of synthesized carbon materials as a biocompatible cosolvent for lysozyme was evaluated. In the case of PB-2 material (synthesized using 0.8:1 biomass to solvent ratio), results show an enhancement of lysozyme activity by 150%. Besides, spectroscopic and calorimetric data confirm the preservation of thermal and structural stability of lysozyme in the PB-2 solution. Thus, this study stipulates PB-2 as an excellent cosolvent for protein studies. With this work, we aim to delve into an entirely new arena of applications of biomass in the field of biotechnology.

中文翻译：

废物生物质向溶剂碳的可持续溶剂热转化：扩展其作为溶菌酶生物相容助溶剂的效用

通过可持续的方法从废物生物质中合成功能性碳材料至关重要，具有广泛的应用。在此，使用公知的溶剂热法合成具有结构变异性的功能性碳材料。通过用酒石酸胆碱（ChBt）和FeCl ₃的混合物处理，将柑桔的剩余纸浆废物生物质（PB）转化为功能碳。（1∶2摩尔比）作为溶剂。在溶剂热处理期间，生物质与溶剂的比率变化为1：1、0.8：1和0.4：1，以分别获得PB-1，PB-2和PB-3作为功能碳材料。通过表征，发现PB碳材料富含具有不同形态的含氧官能团。此外，结果表明溶剂在碳材料合成过程中作为软模板和催化剂的作用。评价了合成碳材料作为溶菌酶生物相容性助溶剂的可行性。对于PB-2材料（使用0.8：1的生物质与溶剂之比合成），结果显示溶菌酶活性提高了150％。此外，光谱和量热数据证实了PB-2溶液中溶菌酶的热稳定性和结构稳定性。从而，这项研究规定PB-2是蛋白质研究的优良助溶剂。通过这项工作，我们旨在深入探讨生物技术在生物技术领域的全新应用领域。

更新日期：2020-09-14

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