ABSTRACT

Objectives: Skeletal muscle adaptation to physical activity is dependent on various factors. Important signaling mediators are reactive oxygen species (ROS). However, recent research suggests that ROS have both beneficial and deleterious effects on exercise adaptation, dependent on training intensity and training status, so that the question of whether anti-oxidants should be taken in connection with exercise cannot easily be answered. Thus, it is important to gain more insight into the complex roles of ROS in regulating training adaptation.

Methods: The effects of ROS inhibition on skeletal muscle training adaptation were analyzed by applying the anti-oxidant PDTC, which is also an inhibitor of the ROS-activated transcription factor nuclear factor kappa B (NFκB), to juvenile mice in connection with a single bout of treadmill running.

Results: We found that PDTC inhibits exercise-mediated induction of specific stress- and inflammation-associated genes. Other genes, specifically those encoding metabolic and mitochondrial factors, were affected to a lesser extent and there appeared to be little effect on the microRNA (miR) profile.

Discussion: Our data suggest that anti-oxidants regulate distinct sets of adaptation-relevant genes, which might have important implications for the design of exercise-based preventive and therapeutic approaches.

摘要

目的：骨骼肌对身体活动的适应取决于多种因素。重要的信号传导介质是活性氧（ROS）。但是，最近的研究表明，ROS对运动适应既有有益作用，也有有害作用，这取决于训练强度和训练状态，因此不能轻易回答是否应在运动中服用抗氧化剂的问题。因此，重要的是要了解ROS在调节训练适应性方面的复杂作用。

方法：通过将抗氧化剂PDTC（也是ROS激活的转录因子核因子κB（NFκB）的抑制剂）应用于与单只小鼠相关的幼年小鼠，分析了ROS抑制对骨骼肌训练适应性的影响。跑步机奔跑。

结果：我们发现PDTC抑制运动介导的特定压力和炎症相关基因的诱导。其他基因，特别是那些编码代谢和线粒体因子的基因，受到的影响较小，对microRNA（miR）谱的影响似乎很小。

讨论：我们的数据表明抗氧化剂调节适应相关基因的不同集合，这可能对设计基于运动的预防和治疗方法具有重要意义。