Jateorhizine (Jat) can reduce blood glucose in diabetic mice, but there are few studies on its role in insulin resistance (IR). This study analyzed the effect of Jat on adipocytes, so as to provide an evidence for the clinical application of Jat. MDI was used to differentiate preadipocytes into adipocytes and induce IR cell models. Different concentrations of Jat (1, 5, 10, 20 μmol/L) were added into undifferentiated and differentiated cells. The cell viability was detected using MTT method. Oil red O staining was performed to observe the lipid formation in cells. Adipolysis method was used to detect the release of glycerol in cell culture medium. The level of 2-DG in cells was detected by glucose uptake assay based on insulin treatment. The expression of adipose transcription factors and IRS2/p-PI3K/p-AKT/GLUT4 signaling pathway was analyzed by western blot (WB) analysis. Neither the activity of differentiated nor undifferentiated preadipocytes was affected by the addition of Jat. There was numerous lipid formation in cells induced by MDI, which was decreased visibly by Jat. Jat reduced the expression levels of MDI-induced elevated levels of PPARγ, C/EBPα, FABP4, perilipin and FAS, as well as increased the release of glycerol in adipocytes. Moreover, Jat further enhanced the 2-DG uptake in MDI-induced adipocytes, and activated the IRS2/p-PI3K/p-AKT/GLUT4 signaling pathway. In general, the role of Jat in adipocytes was concentration-dependent. Jat can not only promote adipolysis, but also increase the glucose uptake in adipocytes, which might be a potential therapy for IR.

Jateorhizine（Jat）可以降低糖尿病小鼠的血糖，但是关于其在胰岛素抵抗（IR）中的作用的研究很少。本研究分析了Jat对脂肪细胞的作用，为Jat的临床应用提供了依据。MDI用于将前脂肪细胞分化为脂肪细胞并诱导IR细胞模型。将不同浓度的Jat（1、5、10、20μmol/ L）加入未分化和分化的细胞中。使用MTT法检测细胞活力。进行油红O染色以观察细胞中脂质的形成。脂肪分解法用于检测甘油在细胞培养基中的释放。通过基于胰岛素处理的葡萄糖摄取测定来检测细胞中2-DG的水平。通过蛋白质印迹（WB）分析来分析脂肪转录因子和IRS2 / p-PI3K / p-AKT / GLUT4信号通路的表达。添加Jat既不影响分化的或未分化的前脂肪细胞的活性。MDI诱导的细胞中有大量脂质形成，Jat明显降低了脂质形成。Jat降低了MDI诱导的PPARγ，C /EBPα，FABP4，周脂蛋白和FAS的表达水平，并增加了甘油在脂肪细胞中的释放。此外，Jat进一步增强了MDI诱导的脂肪细胞对2-DG的摄取，并激活了IRS2 / p-PI3K / p-AKT / GLUT4信号通路。通常，Jat在脂肪细胞中的作用是浓度依赖性的。Jat不仅可以促进脂肪分解，还可以增加脂肪细胞中的葡萄糖摄取，这可能是IR的潜在疗法。