当前位置: X-MOL 学术Anim. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Long-term high-concentrate diet feeding induces apoptosis of rumen epithelial cells and inflammation of rumen epithelium in dairy cows
Animal Biotechnology ( IF 1.7 ) Pub Date : 2020-08-18
Hongyu Dai, NaNa Ma, Guangjun Chang, Zain ul Aabdin, Xiangzhen Shen

The aim of the present study was to evaluate how long-term high-concentrate diet feeding affected rumen epithelium (RE) of dairy cows. So, 12 mid-lactating multiparous cows were divided into two groups randomly fed either with high-concentrate diet (HC, concentrate: forage = 6: 4) or low-concentrate diet (LC, concentrate: forage = 4:6) for 20 weeks. Remarkable upregulation of lipopolysaccharide (LPS) level and depress of pH in rumen fluid were induced by HC compared with LC group. mRNA abundance of interleukin-6 (IL-6), interleukin-8 (IL-8), C-C motif chemokine ligand 5 (CCL5), caspase-3, caspase-8, and caspase-9 were elevated in RE of HC group compared with LC group. Greater protein abundance of phosphorylated NF-κB p65, IL-6, and tumor necrosis factor α (TNF-α) was observed in RE of cows fed HC than that fed LC. Abundance of protein related to proapoptotic response (cytochrome c, BAX and caspase-3) in HC group was greater than that in LC group, while the abundance of anti-apoptotic factor protein (Bcl-2) was lower in HC group than LC group. Therefore, the present study demonstrated that long-term high-concentrate diet feeding upregulated LPS level in rumen fluid and induced the proinflammatory response in the rumen epithelium and apoptosis of rumen epithelial cells.



中文翻译:

长期高浓度饮食喂养可导致奶牛瘤胃上皮细胞凋亡和瘤胃上皮炎症

本研究的目的是评估长期高浓度饮食喂养如何影响奶牛的瘤胃上皮(RE)。因此,将12头泌乳中期的多头母牛分为两组,随机喂养高浓度日粮(HC,浓缩液:草料= 6:4)或低浓度日粮(LC,浓缩液:草料= 4:6),共20头周。HC组与LC组相比,脂多糖(LPS)水平明显升高,瘤胃液pH降低。白细胞介素6IL-6),白细胞介素8IL-8),CC基序趋化因子配体5CCL5),caspase-3caspase-8caspase-9的mRNA丰度HC组的RE较LC组升高。饲喂HC的奶牛的RE中磷酸化的NF-κBp65,IL-6和肿瘤坏死因子α(TNF-α)的蛋白丰度高于饲喂LC的奶牛。HC组细胞凋亡相关蛋白(细胞色素c,BAX和caspase-3)的丰度高于LC组,而抗凋亡因子蛋白(Bcl-2)的丰度低于LC组。 。因此,本研究证明长期高浓度饮食喂养可提高瘤胃液中LPS的水平,并诱导瘤胃上皮的促炎反应和瘤胃上皮细胞的凋亡。

更新日期:2020-08-18
down
wechat
bug