Rapid quantification of Escherichia coli O157:H7 in lettuce and beef using an on‐chip staining microfluidic device,Journal of Food Safety

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Rapid quantification of Escherichia coli O157:H7 in lettuce and beef using an on‐chip staining microfluidic device
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-08-17 , DOI: 10.1111/jfs.12851
Yusuke Tokunaga ₁ , Nobuyasu Yamaguchi ₁

Affiliation

Quality assurance is one of the fundamental ways of preventing infections from foodborne pathogens such as Escherichia coli O157:H7, which produces a deadly toxin. Simple, rapid, and accurate methods for the detection of foodborne pathogens are necessary for healthcare management. In the present study, we applied our microfluidic device, which uses a fluorescent staining‐based detection system, to enumerate E. coli O157:H7 cells in lettuce and beef samples. E. coli O157:H7 cells spiked into lettuce or beef samples were collected using a 0.2‐μm‐pore‐sized filter or a two‐step centrifugation process. The recovery ratios of inoculated E. coli O157:H7 cells from the lettuce and beef samples counted using fluorescence microscopy were 84 (± 10)% and 90 (± 7.3)%, respectively. The counts of E. coli O157:H7 inoculated into lettuce and beef obtained using the microfluidic system were close to the counts obtained using fluorescence microscopy. Our microfluidic approach offers a semi‐automated platform for the quantitative detection of microbial cells from complex food samples and facilitates quantification of microbes in food and food production lines within 1 hr.

中文翻译：

使用片上染色微流控设备对生菜和牛肉中的大肠杆菌O157：H7进行快速定量

质量保证是预防食源性病原体（例如产生致命毒素的大肠杆菌O157：H7）感染的基本方法之一。简单，快速和准确的方法来检测食源性病原体是医疗保健管理所必需的。在本研究中，我们应用了基于荧光染色检测系统的微流控设备，对生菜和牛肉样品中的大肠杆菌O157：H7细胞进行计数。使用0.2μm孔径的过滤器或两步离心过程收集掺入生菜或牛肉样品中的大肠杆菌O157：H7细胞。接种大肠杆菌的回收率使用荧光显微镜对生菜和牛肉样品中的O157：H7细胞的计数分别为84（±10）％和90（±7.3）％。接种到生菜中的大肠杆菌O157：H7的计数和使用微流控系统获得的牛肉的计数接近于使用荧光显微镜获得的计数。我们的微流控方法提供了一个半自动化平台，用于定量检测复杂食品样本中的微生物细胞，并有助于在1小时内对食品和食品生产线中的微生物进行定量。

更新日期：2020-08-17

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