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Peanut (Arachis hypogaea L.) S‐adenosylmethionine decarboxylase confers transgenic tobacco with elevated tolerance to salt stress
Plant Biology ( IF 4.2 ) Pub Date : 2020-08-18 , DOI: 10.1111/plb.13173 D-Y Meng 1 , S Yang 1 , J-Y Xing 2 , N-N Ma 3 , B-Z Wang 2 , F-T Qiu 2 , F Guo 1 , J Meng 1 , J-L Zhang 1 , S-B Wan 4 , X-G Li 1
中文翻译：
花生（Arachis hypergaea L.）S-腺苷甲硫氨酸脱羧酶赋予转基因烟草更高的盐胁迫耐受性
更新日期：2020-08-18
Plant Biology ( IF 4.2 ) Pub Date : 2020-08-18 , DOI: 10.1111/plb.13173 D-Y Meng 1 , S Yang 1 , J-Y Xing 2 , N-N Ma 3 , B-Z Wang 2 , F-T Qiu 2 , F Guo 1 , J Meng 1 , J-L Zhang 1 , S-B Wan 4 , X-G Li 1
Affiliation
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- Polyamines play an important role in stress response. In the pathway of polyamines synthesis, S-adenosylmethionine decarboxylase (SAMDC) is one of the key enzymes. In this study, a full length cDNA of SAMDC (AhSAMDC) was isolated from peanut (Arachis hypogaea L.). Phylogenetic analysis revealed high sequence similarity between AhSAMDC and SAMDC from other plants.
- In peanut seedlings exposed to sodium chloride (NaCl), the transcript level of AhSAMDC in roots was the highest at 24 h that decreased sharply at 72 and 96 h after 150 mM NaCl treatment. However, the expression of AhSAMDC in peanut leaves was significantly inhibited, and the transcript levels in leaves were not different compared with control
- These results implied the tissue‐specific and time-specific expression of AhSAMDC. The physiological effects and functional mechanism of AhSAMDC were further evaluated by overexpressing AhSAMDC in tobaccos. The transgenic tobacco lines exhibited higher germination rate and longer root length under salt stress. Reduced membrane damage, higher antioxidant enzyme activity, and higher proline content were also observed in the transgenic tobacco seedlings. What's more, AhSAMDC also led to higher contents of spermidine and spermine, which can help to scavenge reactive oxygen species.
- Together, this study suggests that AhSAMDC enhances plant resistance to salt stress by improving polyamine content and alleviating membrane damage
中文翻译：
花生（Arachis hypergaea L.）S-腺苷甲硫氨酸脱羧酶赋予转基因烟草更高的盐胁迫耐受性
多胺在应激反应中发挥重要作用。在多胺合成途径中，S-腺苷甲硫氨酸脱羧酶（SAMDC）是关键酶之一。本研究从花生（ ArachishypogaeaL .）中分离得到SAMDC （ AhSAMDC ）的全长cDNA。系统发育分析揭示了AhSAMDC和来自其他植物的SAMDC之间的高度序列相似性。
在暴露于氯化钠（NaCl）的花生幼苗中，根中AhSAMDC的转录水平在24小时时最高，并在150 mM NaCl处理后的72和96小时急剧下降。但花生叶片中AhSAMDC的表达受到显着抑制，且叶片中的转录水平与对照相比没有差异。
这些结果暗示了AhSAMDC的组织特异性和时间特异性表达。通过在烟草中过表达AhSAMDC，进一步评估了AhSAMDC的生理效应和功能机制。转基因烟草品系在盐胁迫下表现出更高的发芽率和更长的根长。在转基因烟草幼苗中还观察到膜损伤减少、抗氧化酶活性更高、脯氨酸含量更高。更重要的是，AhSAMDC还导致亚精胺和精胺的含量更高，这有助于清除活性氧。
总之，这项研究表明 AhSAMDC 通过提高多胺含量和减轻膜损伤来增强植物对盐胁迫的抵抗力
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