Long noncoding RNAs (lncRNAs) can act as oncogene and tumor suppressor genes in many types of cancers including breast cancer (BC). Our previous study has indicated microRNA (miR)‐125a‐5p was downregulated and function as a tumor suppressor in BC. However, its upstream regulation mechanism is still unclear. In this study, we used bioinformatics algorithms, RNA pulldown assay, and dual‐luciferase reports assay to predict and confirm lncRNA CERS6‐AS1 interacted with miR‐125a‐5p. Then we found CERS6‐AS1 was upregulated in BC tissues. Experimental results of tumor growth in nude mice show that CERS6‐AS1 promotes tumor growth. Furthermore, CERS6‐AS1 regulated BC susceptibility gene 1‐associated protein 1 (BAP1) expression via sponging miR‐125a‐5p via Western blot analysis and quantitative polymerase chain reaction arrays. Finally, we showed that miR‐125a‐5p had opposing effects to those of CERS6‐AS1 on BC cells, demonstrating that CERS6‐AS1 may promote cell proliferation and inhibit cell apoptosis via sponging miR‐125a‐5p. Our results indicated CERS6‐AS1 promote BC cell proliferation and inhibit cell apoptosis via sponging miR‐125a‐5p to upregulate BAP1 expression.

中文翻译：

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lncRNA CERS6-AS1 作为 ceRNA 通过海绵化 miR-125a-5p 上调 BAP1 表达来促进乳腺癌细胞增殖。

长链非编码 RNA (lncRNA) 可以在包括乳腺癌 (BC) 在内的多种癌症中充当致癌基因和抑癌基因。我们之前的研究表明，microRNA (miR)-125a-5p 在 BC 中被下调并作为肿瘤抑制因子发挥作用。但其上游调控机制尚不明确。在这项研究中，我们使用生物信息学算法、RNA pulldown 分析和双荧光素酶报告分析来预测和确认 lncRNA CERS6-AS1 与 miR-125a-5p 相互作用。然后我们发现 CERS6-AS1 在 BC 组织中被上调。裸鼠肿瘤生长实验结果表明，CERS6-AS1促进肿瘤生长。此外，CERS6-AS1 通过蛋白质印迹分析和定量聚合酶链反应阵列通过海绵化 miR-125a-5p 调节 BC 易感基因 1 相关蛋白 1 (BAP1) 的表达。最后，我们发现 miR-125a-5p 对 BC 细胞具有与 CERS6-AS1 相反的作用，表明 CERS6-AS1 可通过海绵作用 miR-125a-5p 促进细胞增殖并抑制细胞凋亡。我们的结果表明，CERS6-AS1 通过海绵化 miR-125a-5p 上调 BAP1 表达来促进 BC 细胞增殖并抑制细胞凋亡。