Thermoresponsive Cationic Block Copolymer Brushes for Temperature-Modulated Stem Cell Separation.,Macromolecular Rapid Communications

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Thermoresponsive Cationic Block Copolymer Brushes for Temperature-Modulated Stem Cell Separation.
Macromolecular Rapid Communications ( IF 4.2 ) Pub Date : 2020-08-17 , DOI: 10.1002/marc.202000308
Kenichi Nagase ₁ , Ayumu Ota ₁ , Tadashi Hirotani ₁ , Sota Yamada ₁ , Aya Mizutani Akimoto ₂ , Hideko Kanazawa ₁

Affiliation

Recently, cell separation methods have become important for preparing cells for transplantation therapy. In this study, a thermoresponsive cationic block copolymer brush is developed as an effective cell separation tool. This brush is prepared on glass surfaces using two steps of activator regenerated by electron transfer–atom transfer radical polymerization (ARGET‐ATRP). The cationic segment is prepared in the first step of the ARGET‐ATRP of N,N‐dimethylaminopropylacrylamide (DMAPAAm). In the second step, the thermoresponsive segment is prepared, attached to the bottom cationic segment, through ARGET‐ATRP with N‐isopropylacrylamide (NIPAAm). The cell adhesion behavior of the prepared thermoresponsive cationic copolymer, PDMAPAAm‐b‐PNIPAAm, brush is observed using umbilical cord‐derived mesenchymal stem cells (UCMSC), fibroblasts, and macrophages. At 37 °C, all three types of cells adhere to the thermoresponsive cationic copolymer brush. Then, by reducing the temperature to 20 °C, the adhered UCMSC are detached from the copolymer brush, whereas the fibroblasts and macrophages remain adhered to the copolymer brush. Using this copolymer brush, UCMSC can be purified from the cell mixture simply by changing the temperature. Therefore, the prepared thermoresponsive cationic copolymer brush is useful as a cell separation tool for the purification of mesenchymal stem cells.

中文翻译：

用于温度调节干细胞分离的热敏阳离子嵌段共聚物刷。

近来，细胞分离方法对于制备用于移植治疗的细胞已变得重要。在这项研究中，开发了一种热响应性阳离子嵌段共聚物刷作为一种有效的细胞分离工具。该刷子是在玻璃表面上使用通过电子转移再生的两步活化剂–原子转移自由基聚合（ARGET-ATRP）制备的。阳离子链段是在N，N-二甲基氨基丙基丙烯酰胺（DMAPAAm）的ARGET-ATRP的第一步中制备的。在第二步中，通过带有N-异丙基丙烯酰胺（NIPAAm）的ARGET-ATRP，制备与底部阳离子段相连的热敏段。所制备的热响应阳离子共聚物，PDMAPAAm-的细胞粘附行为b‐PNIPAAm，使用脐带间充质干细胞（UCMSC），成纤维细胞和巨噬细胞进行刷洗。在37°C下，所有三种类型的电池均粘附在热响应性阳离子共聚物刷上。然后，通过将温度降低至20°C，将粘附的UCMSC从共聚物刷上取下，而成纤维细胞和巨噬细胞仍粘附在共聚物刷上。使用该共聚物刷，只需更改温度即可从细胞混合物中纯化UCMSC。因此，制备的热响应性阳离子共聚物刷可用作细胞分离工具，用于纯化间充质干细胞。

更新日期：2020-10-05

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