Ark shell protein-derived bioactive peptides promote osteoblastic differentiation through upregulation of the canonical Wnt/β-catenin signaling in human bone marrow-derived mesenchymal stem cells.,Journal of Food Biochemistry

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Ark shell protein-derived bioactive peptides promote osteoblastic differentiation through upregulation of the canonical Wnt/β-catenin signaling in human bone marrow-derived mesenchymal stem cells.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-08-18 , DOI: 10.1111/jfbc.13440
Yunok Oh ₁ , Chang-Bum Ahn ₂ , Jae-Young Je ₃

Affiliation

In this study, the stimulating effect of ark shell protein‐derived peptides AWLNH and PHDL on osteoblast differentiation in human bone marrow‐derived mesenchymal stem cells (hBMMSCs) and its molecular mechanism was investigated. The hBMMSCs were cultured with two peptides and osteogenic markers were analyzed. Results showed that enhanced ALP activity and calcification were detected in the presence of AWLNH and PHDL. Based on western blotting, RT‐qPCR, and immunostaining analysis, AWLNH and PHDL are specific for osteoblast differentiation of hBMMSCs through activating the canonical Wnt/β‐catenin signaling pathway followed by activating Runx2, osterix, and type I collagen. Loss‐of‐function assay with DKK‐1, a Wnt antagonist, showed that the canonical Wnt/β‐catenin signaling was essential for AWLNH and PHDL‐induced osteogenesis in hBMMSCs. These findings suggested that AWLNH and PHDL can stimulate osteoblast differentiation of hBMMSCs via upregulating the canonical Wnt/β‐catenin signaling and may be useful for a potential nutraceuticals or pharmaceuticals to treat osteoporosis.

中文翻译：

方舟壳蛋白衍生的生物活性肽通过上调人骨髓间充质干细胞中的经典 Wnt/β-连环蛋白信号传导来促进成骨细胞分化。

本研究探讨了方舟壳蛋白衍生肽AWLNH和PHDL对人骨髓间充质干细胞（hBMMSCs）成骨细胞分化的刺激作用及其分子机制。用两种肽培养 hBMMSC，并分析成骨标志物。结果表明，在 AWLNH 和 PHDL 存在的情况下，检测到 ALP 活性和钙化增强。基于蛋白质印迹、RT-qPCR 和免疫染色分析，AWLNH 和 PHDL 通过激活经典 Wnt/β-catenin 信号通路，然后激活 Runx2、osterix 和 I 型胶原蛋白，对 hBMMSC 的成骨细胞分化具有特异性。使用 Wnt 拮抗剂 DKK-1 进行的功能丧失测定表明，经典的 Wnt/β-catenin 信号传导对于 AWLNH 和 PHDL 诱导的 hBMMSC 成骨至关重要。这些发现表明，AWLNH 和 PHDL 可以通过上调典型的 Wnt/β-catenin 信号传导来刺激 hBMMSC 的成骨细胞分化，并且可能有助于成为治疗骨质疏松症的潜在营养保健品或药物。

更新日期：2020-10-11

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