The membrane‐associated mucin (MAM) domain containing glycosylphosphatidylinositol anchor 2 protein single knock‐out mice (MDGA2^+/−) are models of ASD. We examined the behavioral phenotypes of male and female MDGA2^+/− and wildtype mice on C57BL6/NJ and C57BL6/N backgrounds at 2 months of age and measured MDGA2, neuroligin 1 and neuroligin 2 levels at 7 months. Mice on the C57BL6/NJ background performed better than those on the C57BL6/N background in visual ability and in learning and memory performance in the Morris water maze and differed in measures of motor behavior and anxiety. Mice with the MDGA2^+/− genotype differed from WT mice in motor, social and repetitive behavior and anxiety, but most of these effects involved interactions between MDGA2^+/− genotype and background strain. The background strain also influenced MDGA2 levels and NLGN2 association in MDGA2^+/− mice. Our findings emphasize the importance of the background strain used in studies of genetically modified mice.

中文翻译：

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背景应变对自闭症谱系障碍MDGA2+/-小鼠模型行为表型的影响

含有糖基磷脂酰肌醇锚定 2 蛋白单敲除小鼠 (MDGA2 ^+/- ) 的膜相关粘蛋白 (MAM) 结构域是 ASD 的模型。我们在 2 个月大时检查了雄性和雌性 MDGA2 ^+/-和野生型小鼠在 C57BL6/NJ 和 C57BL6/N 背景下的行为表型，并在 7 个月时测量了 MDGA2、neuroligin 1 和 neuroligin 2 水平。在莫里斯水迷宫中，C57BL6/NJ 背景的小鼠在视觉能力和学习和记忆能力方面的表现优于 C57BL6/N 背景的小鼠，并且在运动行为和焦虑的测量方面存在差异。具有 MDGA2 ^+/-基因型的小鼠在运动、社交和重复行为以及焦虑方面与 WT 小鼠不同，但大多数这些影响涉及 MDGA2 之间的相互作用^+/-基因型和背景菌株。背景菌株也影响 MDGA2 ^+/-小鼠中的 MDGA2 水平和 NLGN2 关联。我们的研究结果强调了转基因小鼠研究中使用的背景菌株的重要性。