Cell‐Membrane Staining Properties and Photocytotoxicity of a Ruthenium(II) Photosensitizer,European Journal of Inorganic Chemistry

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Cell‐Membrane Staining Properties and Photocytotoxicity of a Ruthenium(II) Photosensitizer
European Journal of Inorganic Chemistry ( IF 2.2 ) Pub Date : 2020-08-17 , DOI: 10.1002/ejic.202000702
Kenneth Yin Zhang ₁ , Linna Song ₁ , Tianhan Gu ₁ , Hao Wang ₁ , Chao Yang ₁ , Hanchen Zhou ₁ , Pengli Gao ₁ , Shujuan Liu ₁ , Qiang Zhao ₁

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Organelle‐targeting photosensitizers have attracted increasing attention in the development of drugs for photodynamic therapy because they can efficiently induce cellular functional disorder upon light irradiation. In this work, we reported a ruthenium(II) complex bearing two lipophilic carbon chains which specifically binds to the cell membrane. Light irradiation on the complex‐treated cells caused an increase of intracellular reactive oxygen species. The photocytotoxicity of the complex toward HeLa cells were evaluated via the 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. A high dose (3 µm) of the complex induced apoptotic cell death by photogeneration of intracellular reactive oxygen species. A low dose (0.25 µm) of the complex did not cause noticeable cell death but increased the permeability of the cell membrane upon light irradiation.

中文翻译：

钌（II）光敏剂的细胞膜染色特性和光细胞毒性

靶向细胞器的光敏剂在光动力疗法药物的开发中引起了越来越多的关注，因为它们可以在光照射下有效诱导细胞功能紊乱。在这项工作中，我们报道了带有两个亲脂性碳链的钌（II）络合物，它特异性地结合到细胞膜上。复合物处理过的细胞上的光照射引起细胞内活性氧种类的增加。通过3-（4,5-二甲基-2-噻唑基）-2-溴5-二苯基四唑（MTT）分析评估了复合物对HeLa细胞的光细胞毒性。高剂量（3 µm）的复合物通过细胞内活性氧物质的光生作用诱导凋亡细胞死亡。低剂量（0.25 µm）的配合物不会引起明显的细胞死亡，但是在光照射下增加了细胞膜的通透性。

更新日期：2020-08-17

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