The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are urgently needed to manage and control the spread of SARS-CoV-2. RT-qPCR based methods are the gold standard for SARS-CoV-2 detection. However, these methods require trained personnel, sophisticated infrastructure, and a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), a one-step nucleic acid amplification method conducted at a single temperature, has been used for colorimetric virus detection. CRISPR-Cas12 and CRISPR-Cas13 systems, which possess collateral activity against ssDNA and RNA, respectively, have also been harnessed for virus detection. Here, we built an efficient, rapid, specific, sensitive, user-friendly SARS-CoV-2 detection module that combines the robust virus amplification of RT-LAMP with the specific detection ability of SARS-CoV-2 by CRISPR-Cas12. Furthermore, we combined the RT-LAMP-CRISPR-Cas12 module with lateral flow cells to enable highly efficient point-of-care SARS-CoV-2 detection. Our iSCAN SARS-CoV-2 detection module, which exhibits the critical features of a robust molecular diagnostic device, should facilitate the effective management and control of COVID-19.

SARS-CoV-2引起的COVID-19大流行影响人类生活的各个方面。迫切需要高效，快速，准确，特异，敏感和用户友好的检测平台来管理和控制SARS-CoV-2的传播。基于RT-qPCR的方法是SARS-CoV-2检测的金标准。但是，这些方法需要训练有素的人员，复杂的基础结构以及较长的周转时间，从而限制了其实用性。反转录环介导的等温扩增（RT-LAMP）是在单一温度下进行的一步式核酸扩增方法，已用于比色病毒检测。CRISPR-Cas12和CRISPR-Cas13系统分别具有针对ssDNA和RNA的附带活性，也已被用于病毒检测。在这里，我们建立了高效，快速，具体，灵敏，用户友好的SARS-CoV-2检测模块，结合了RT-LAMP强大的病毒扩增能力和CRISPR-Cas12对SARS-CoV-2的特异性检测能力。此外，我们将RT-LAMP-CRISPR-Cas12模块与侧向流通池结合使用，以实现高效的即时医疗SARS-CoV-2检测。我们的iSCAN SARS-CoV-2检测模块具有强大的分子诊断设备的关键功能，应该可以有效地管理和控制COVID-19。