To evaluate the nanoparticle (NP) toxicity, much efforts have been devoted for developing methods to accurately disperse NPs into aqueous suspensions prior to in vitro toxicological studies. As NP toxicity is strongly dependent on their physicochemical properties, NP characterization is a key step for any in vitro toxicological study. This study demonstrates that the static multiple light scattering (SMLS) technique allows for the simultaneous screening of the NP size, agglomeration state, stability and dosimetry in biological media. Batch dispersions of TiO₂ P25 NPs in water with various bovine serum albumin (BSA) mass fractions (from 0% to 0.5%) and dilutions of these dispersions into cell culture media were characterized with SMLS. In the batch dispersions, TiO₂ NPs are stable and well dispersed for BSA mass fraction lower than 0.2% while agglomeration and rapid settling is observed for higher BSA mass fractions. Paradoxically, when diluted in cell culture media, TiO₂ NPs are well dispersed and stable for BSA mass fractions higher than 0.2%. The TiO₂ NP dosimetry of these dilutions was evaluated experimentally with SMLS and confronted with numerical approaches. The TiO₂ NP bottom concentration evolves far more slowly in the case of the higher BSA mass fraction. Such measurements give valuable insights on the NP fate and transport in biological media to obtain in fine reliable size and dose-cytotoxicity responses.

为了评估纳米颗粒 (NP) 的毒性，在体外毒理学研究之前，已经致力于开发将纳米颗粒准确分散到水悬浮液中的方法。由于 NP 毒性强烈依赖于它们的理化特性，NP 表征是任何体外毒理学研究的关键步骤。这项研究表明，静态多重光散射 (SMLS) 技术允许同时筛选生物介质中的 NP 尺寸、团聚状态、稳定性和剂量学。使用 SMLS 表征具有各种牛血清白蛋白 (BSA) 质量分数（从 0% 到 0.5%）的 TiO ₂ P25 NPs 在水中的批量分散体以及这些分散体在细胞培养基中的稀释。在分批分散体中，TiO ₂对于低于 0.2% 的 BSA 质量分数，NPs 稳定且分散良好，而对于较高的 BSA 质量分数，观察到团聚和快速沉降。矛盾的是，当在细胞培养基中稀释时，TiO ₂ NPs 分散良好并且对于高于 0.2% 的 BSA 质量分数是稳定的。这些稀释液的 TiO ₂ NP 剂量测定是用 SMLS 实验评估的，并面临数值方法。在较高 BSA 质量分数的情况下，TiO ₂ NP 底部浓度的变化要慢得多。这样的测量为生物介质中 NP 的命运和运输提供了有价值的见解，以获得精确可靠的大小和剂量-细胞毒性反应。