Abstract 17β-Estradiol (17β-E2) is the reproductive hormone playing a major role in female reproductive system and acts as an important component of metabolic processes. Quantifying 17β-E2 level helps to identify the reproductive endocrine related problems. Herein, a salt-induced (from 60 mM) gold nanoparticle (GNP) based immunoassay was introduced to quantify 17β-E2. Polyclonal antibody (50 nM) was immobilized on the surface of GNP and the color with GNPs was changed (blue to red shift) upon adding the target in the presence of high monovalent salt (NaCl) concentration. The sensitivity was at 10 pM with the point of GNPs assembly to disassembly. Negative control experiments did not show any color changes with GNPs indicate the specific detection of 17β-E2. In addition, selective detection was proved in the presence of other organic-, inorganic-, biological-molecules and the serum. This simple bare eye detection is quantifying the level of 17β-E2 and diagnosing the reproductive endocrine related disorders.

中文翻译：

---

金纳米颗粒在比色免疫分析中的组装和拆卸以检测 17β-雌二醇并确定妇科疾病

摘要 17β-雌二醇（17β-E2）是一种在女性生殖系统中起主要作用的生殖激素，是代谢过程的重要组成部分。量化 17β-E2 水平有助于识别生殖内分泌相关问题。在此，引入了盐诱导（来自 60 mM）金纳米颗粒 (GNP) 的免疫测定来量化 17β-E2。多克隆抗体 (50 nM) 固定在 GNP 的表面，在高单价盐 (NaCl) 浓度存在下添加目标后，GNP 的颜色发生变化（蓝移至红移）。灵敏度为 10 pM，具有 GNP 组装到拆卸的点。阴性对照实验未显示 GNP 的任何颜色变化表明 17β-E2 的特异性检测。此外，在其他有机-、无机-、生物分子和血清。这种简单的裸眼检测正在量化 17β-E2 的水平并诊断生殖内分泌相关疾病。