TEB belongs to the family of triazole fungicides and it is used to protect agricultural crop plants from fungal pathogens. The information regarding its cardiotoxic effects through different pathways particularly by perturbing the oxidative balance and causing damage to the myocardium is still limited. In the present study, oxidative and histopathologic damages caused by TEB in the cardiac tissue of male adult rats, were evaluated. Rats were exposed orally to TEB at 0.9, 9, 27 and 45 mg/kg b.w. for 28 days. Results showed that following TEB treatment malondialdehyde (MDA), protein carbonyl (PC), advanced oxidation protein product (AOPP), antioxidant enzyme activities (GPx and GR) and GSSG levels increased, while GSH levels and thus the GSH/GSSG ratio decreased. Superoxide dismutase (SOD) and catalase (CAT) initially increased at the doses of 0.9, 9 and 27 mg/kg b.w. and then decreased at the dose of 45 mg/kg b.w. Moreover, western blot analysis showed that TEB increased SOD1, CAT and HSP70 protein levels after 24 h. Furthermore, TEB induced various histological changes in the myocardium, including leucocytic infiltration, hemorrhage congestion of cardiac blood vessels and cytoplasmic vacuolization. Therefore, our investigation revealed, that TEB exhibits cardiotoxic effects by changing oxidative balance and damaging the cardiac tissue.

中文翻译：

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戊唑醇诱导成年大鼠心脏氧化应激和组织病理学改变

TEB 属于三唑类杀菌剂家族，用于保护农作物免受真菌病原体的侵害。关于其通过不同途径，特别是通过扰乱氧化平衡和对心肌造成损害的心脏毒性作用的信息仍然有限。在本研究中，评估了 TEB 在雄性成年大鼠心脏组织中引起的氧化和组织病理学损伤。大鼠口服暴露于 0.9、9、27 和 45 毫克/千克体重的 TEB，持续 28 天。结果表明，TEB处理后丙二醛（MDA）、蛋白羰基（PC）、高级氧化蛋白产物（AOPP）、抗氧化酶活性（GPx和GR）和GSSG水平增加，而GSH水平和GSH/GSSG比值降低。超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 最初在剂量为 0.9 时增加，9 和 27 mg/kg bw，然后在 45 mg/kg bw 的剂量下降低 此外，蛋白质印迹分析表明，TEB 在 24 小时后增加了 SOD1、CAT 和 HSP70 蛋白水平。此外，TEB 诱导心肌的各种组织学变化，包括白细胞浸润、心脏血管出血充血和细胞质空泡化。因此，我们的研究表明，TEB 通过改变氧化平衡和破坏心脏组织表现出心脏毒性作用。