An electrochemical biosensor containing Co-hemin metal organic frameworks (Co-hemin MOF)/chitosan composite and cellobiose dehydrogenase (CDH) was developed for lactose dectection. The formation of the Co-hemin MOF was verified by scanning electron microscopy, Fourier transform infrared spectroscopy, UV–vis absorption spectroscopy and electrochemical techniques. The Co-hemin MOF/chitosan composite exhibited outstanding electrochemical performance on lactose detection with high sensitivity (102.3 μA mM⁻¹ cm⁻²) and fast response (5 s). More importantly, the lower determination limit of 4 mM and the broad linear ranges of 10 to 100 mM were obtained to detect lactose. With broad linear ranges, the feasibility of dairy application of the biosensor based on Co-hemin MOF electrode was demonstrated in this study.

电化学生物传感器包含Co-hemin金属有机框架（Co-hemin MOF）/壳聚糖复合材料和纤维二糖脱氢酶（CDH）用于乳糖检测。通过扫描电子显微镜，傅立叶变换红外光谱，紫外可见吸收光谱和电化学技术验证了共-血红素MOF的形成。Co-hemin MOF /壳聚糖复合物在乳糖检测中表现出出色的电化学性能，灵敏度高（102.3μAmM ^-1 cm ^-2）和快速响应（5 s）。更重要的是，检测乳糖的测定下限为4 mM，线性范围为10至100 mM。在宽线性范围内，本研究证明了基于Co-hemin MOF电极的生物传感器在乳制品中应用的可行性。