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Physiological changes in the hemolymph of juvenile shrimp Litopenaeus vannamei to sublethal nitrite and nitrate stress in low-salinity waters.
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-08-18 , DOI: 10.1016/j.etap.2020.103472
Gladys Valencia-Castañeda 1 , Martín G Frías-Espericueta 2 , Ruth C Vanegas-Pérez 3 , María C Chávez-Sánchez 4 , Federico Páez-Osuna 5
Affiliation  

Juveniles of the shrimp Litopenaeus vannamei (3.3 ± 0.4 g) were exposed separately to nitrite (0.0, 1.1, 2.6, and 5.3 mg/L nitrogen as nitrite [NO2-N]) and nitrate (0, 90, 225 and 400 mg/L nitrogen as nitrate [NO3-N]) concentrations equivalent to 0, 10, 25, and 50% of the LC50-96 h value of NO2-N and NO3-N in low salinity water (3 g/L). Shrimps responded to nitrite and nitrate according to changes in oxyhemocyanin, glucose, lactate and ion levels in the hemolymph after 6, 12, 24, and 48 h of exposure. Oxyhemocyanin levels decreased with increasing nitrite and nitrate levels and were higher at 50% exposure to the contaminants. Compared to the control, glucose and lactate increased significantly at 50% exposure to nitrite and nitrate, particularly at 12 and 24 h. Na+ in the hemolymph changed with nitrite and nitrate, while K+ only changed ˜with nitrite.



中文翻译:

低盐度海水中凡纳滨对虾对虾亚硝酸盐致死和硝酸盐胁迫的生理变化。

南美白对虾(3.3±0.4 g)的幼鱼分别暴露于亚硝酸盐(0.0、1.1、2.6和5.3 mg / L的亚硝酸盐形式[NO 2 -- N])和硝酸盐(0、90、225和400)毫克/升氮作为硝酸盐[NO 3 - -N])的浓度相当于0,10,25,和LC 50%50 NO -96 H值为2 - -N和NO 3 --N在低盐度水中(3 g / L)。在暴露6、12、24和48小时后,虾根据血红素中氧合血色素,葡萄糖,乳酸和离子水平的变化对亚硝酸盐和硝酸盐作出反应。氧合血红素水平随着亚硝酸盐和硝酸盐水平的增加而降低,在接触污染物50%时更高。与对照组相比,葡萄糖和乳酸盐在50%暴露于亚硝酸盐和硝酸盐时显着增加,尤其是在12和24小时。血淋巴中的Na +随亚硝酸盐和硝酸盐而变化,而K +仅随亚硝酸盐而变化。

更新日期:2020-08-18
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