The expression of genes related to the Toll-like receptors (TLRs) signaling pathway were determined.

Group A, B and C fed with basal diet and group D, E and F induced TD by feeding a basal diet containing 100 mg·kg⁻¹ thiram. rGSTA3 protein was injected at 20 μg·kg⁻¹ in group B, E and at 50 μg·kg⁻¹ in C, F.

Results suggested that lameness and death of chondrocytes were significant on day 14. TLRs signaling pathway related genes were screened based on the transcriptome enrichment, and validated on qPCR. IL-7, TLR2, 3, 4, 5, 7, 15, MyD88, MHC-II, MDA5 and TRAF6 were significantly (p < 0.05) expressed in group E and F as compared to group D on day 14 and 23. IL-7, MHCII, TRAF6, TLR3, TLR5, TLR7, and TLR15 determined insignificant in group D compared to group A on day 23.

TD occur in an early phase and alleviated in the later period. rGSTA3 protein can prevent apoptosis and repair degraded chondrocytes.

测定了与 Toll 样受体 (TLR) 信号通路相关的基因的表达。

A、B、C组饲喂基础饲料，D、E、F组饲喂含100 mg·kg ^-1福美双的基础饲料诱导TD。 B、E组注射rGSTA3蛋白20 μg·kg ^-1 ，C、F组注射rGSTA3蛋白50 μg·kg ^-1 。

结果表明，第14天软骨细胞跛行和死亡显着。根据转录组富集筛选TLRs信号通路相关基因，并通过qPCR进行验证。第 14 天和第 23 天，与 D 组相比，E 组和 F 组中 IL-7、TLR2、3、4、5、7、15、MyD88、MHC-II、MDA5 和 TRAF6 显着表达（ p < 0.05）。第 23 天，D 组中的 -7、MHCII、TRAF6、TLR3、TLR5、TLR7 和 TLR15 与 A 组相比不显着。

TD早期发生，后期减轻。 rGSTA3蛋白可以防止细胞凋亡并修复退化的软骨细胞。