The efficient carrier design for transferring therapeutic genes into target cells as well as tracking the delivered agents has attracted lots of attention in the field of DNA-based therapeutics. Here, we demonstrate this concept by a fast and facilitated method using BSA gold nanocluster (BSA AuNcs) conjugated with chimeric peptide with ability of DNA binding/packaging, endosome disruption and cell nuclear localization. An extensive characterization of photoluminescence properties, electrophoresis mobility and size distribution of the nanocarrier demonstrating the stable complexes composed of plasmid DNA, chimeric peptide and BSA AuNcs were successfully formed through electrostatic interactions. In the hybrid complexes, chimeric peptide could effectively decrease the cytotoxicity of AuNcs as well as enhance internalization of plasmid harboring firefly luciferase gene into HEK 293 T. The designed nanocarrier could be a promising vector in gene delivery systems for improved theranostics applications.

用于将治疗性基因转移到靶细胞以及跟踪所输送药剂的有效载体设计在基于DNA的治疗剂领域引起了很多关注。在这里，我们通过使用BSA金纳米团簇（BSA AuNcs）与具有DNA结合/包装，内体破坏和细胞核定位能力的嵌合肽共轭的快速简便方法，证明了这一概念。通过静电相互作用成功地形成了纳米载体的光致发光性质，电泳迁移率和大小分布的广泛表征，证明了由质粒DNA，嵌合肽和BSA AuNcs组成的稳定复合物。在杂合体中