We optimized the preparation of tannylated-human serum albumin (TA-HSA) nanoagents including indocyanine green (ICG) and lobeglitazone (Lobe)-loaded complexes by simple mixing and characterized them. We set the optimal pH at 5 and the optimal weight ratio of [TA]/[HSA] at 1.5 when considering the redispersion of the prepared suspensions in PBS (pH 7.4) and incubation at 37 ·C for the preparation of ICG and/or Lobe-loaded TA-HSA complexes. The immobilization of methoxy polyethylene glycol (mPEG) molecules on TA-HSA-ICG complexes significantly reduces the mean and Z-average sizes of these complexes from the microscale to the nanoscale. Moreover, the use of ICG increases the drug loading efficiency when preparing mPEG-TA-HSA-ICG/Lobe.

我们通过简单混合优化了载有吲哚花青绿（ICG）和洛贝格列酮（Lobe）的复合物的坦尼化人血清白蛋白（TA-HSA）纳米剂的制备，并对其进行了表征。考虑到制备的悬浮液在PBS中的重新分散（pH 7.4）并在37°C孵育以制备ICG和/或，我们将最佳pH设置为5，将[TA] / [HSA]的最佳重量比设置为1.5。装有凸耳的TA-HSA复合物。将甲氧基聚乙二醇（mPEG）分子固定在TA-HSA-ICG配合物上，可从微米级到纳米级显着降低这些配合物的平均尺寸和Z平均尺寸。此外，在制备mPEG-TA-HSA-ICG / Lobe时，ICG的使用可提高药物装载效率。