Ammonia is used as a fertilizer for agriculture, chemical raw material, and carrier for transporting hydrogen, and with economic development, the demand for ammonia has increased. The Haber–Bosch process, which is the main method for producing ammonia, can produce ammonia with high efficiency. However, since it consumes a large amount of fossil energy, it is necessary to develop an alternative method for producing ammonia with less environmental impact. Ammonia production from food by-products is an appealing production process owing to unused resource usage, including waste, and mild reaction conditions. However, when food by-products and biomass are used as feedstocks, impurities often reduce productivity. Using metabolic profiling, glucose was identified as a potential inhibitor of ammonia production from impure food by-products. We constructed the recombinant Escherichia coli, in which glucose uptake was reduced by ptsG gene disruption and amino acid catabolism was promoted by glnA gene disruption. Ammonia production efficiency from okara, a food by-product, was improved in this strain; 35.4 mM ammonia was produced (47% yield). This study might provide a strategy for efficient ammonia production from food by-products.

中文翻译：

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通过工程化大肠杆菌从食品副产品中高效生产氨。

氨被用作农业肥料，化学原料和氢的运输载体，并且随着经济发展，对氨的需求增加了。哈伯-博世（Haber-Bosch）工艺是生产氨的主要方法，可以高效生产氨。然而，由于其消耗大量的化石能量，因此有必要开发一种对环境影响较小的生产氨的替代方法。食品副产品生产氨是一个有吸引力的生产过程，因为未使用的资源（包括废物）和反应条件温和。但是，当将食品副产品和生物质用作原料时，杂质通常会降低生产率。使用代谢谱分析，葡萄糖被鉴定为潜在的抑制不纯食品副产品生产氨的方法。大肠杆菌，其中通过ptsG基因破坏降低了葡萄糖的摄取，而通过glnA基因破坏促进了氨基酸的分解代谢。食品副产品豆渣的氨生产效率在该菌株中得到了提高。产生35.4mM氨（47％产率）。这项研究可能为从食品副产物中高效生产氨提供一种策略。