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Do acute hepatopancreatic necrosis disease-causing PirABVP toxins aggravate vibriosis?
Emerging Microbes & Infections ( IF 8.4 ) Pub Date : 2020-09-02 , DOI: 10.1080/22221751.2020.1811778
Phuong Thi Ngoc Tran 1 , Vikash Kumar 1, 2 , Peter Bossier 1
Affiliation  

Gram-negative marine bacterium Vibrio parahaemolyticus is an important aquatic pathogen and has been demonstrated to be the causative agent of acute hepatopancreatic necrotic disease (AHPND) in shrimp aquaculture. The AHPND-causing V. parahaemolyticus strains contain a pVA1 plasmid encoding the binary PirAVP and PirBVP toxins, are the primary virulence factor that mediates AHPND and mortality in shrimp. Since PirABVP toxins are secreted extracellularly, one can hypothesize that PirABVP toxins would aggravate vibriosis in the aquatic environment. To address this, in vivo and in vitro experiments were conducted. Germ-free Artemia franciscana were co-challenged with PirABVP toxins and 10 Vibrio spp. The in vivo results showed that PirABVP toxin interact synergistically with MM30 (a quorum sensing AI-2 deficient mutant) and V. alginolyticus AQ13-91, aggravating vibriosis. However, co-challenge by PirABVP toxins and V. campbellii LMG21363, V. parahaemolyticus CAIM170, V. proteolyticus LMG10942, and V. anguillarum NB10 worked antagonistically, increasing the survival of Artemia larvae. The in vitro results showed that the addition of PirABVP toxins significantly modulated the production of the virulence factors of studied Vibrio spp. Yet these in vitro results did not help to explain the in vivo results. Hence it appears that PirABVP toxins can aggravate vibriosis. However, the dynamics of interaction is strain dependent.



中文翻译:

引起急性肝胰腺坏死病的 PirABVP 毒素会加重弧菌病吗?

革兰氏阴性海洋细菌副溶血性弧菌是一种重要的水生病原体,已被证明是对虾养殖中急性肝胰腺坏死病 (AHPND) 的病原体。引起 AHPND 的副溶血性弧菌菌株含有编码二元 PirA VP和 PirB VP毒素的 pVA1 质粒,是介导 AHPND 和虾死亡率的主要毒力因子。由于 PirAB VP毒素在细胞外分泌,因此可以假设 PirAB VP毒素会加剧水生环境中的弧菌病。为了解决这个问题,进行了体内体外实验。无菌卤虫与 PirAB VP毒素和 10种弧菌共同攻击。该体内结果显示,PirAB VP毒素与MM30协同相互作用(一群体感应AI-2缺陷型突变)和溶藻弧菌AQ13-91,加重弧菌病。然而,PirAB共同挑战VP毒素和五,坎LMG21363,副溶血弧菌CAIM170,溶蛋白弧菌LMG10942和鳗弧菌NB10工作对抗性,提高生存的卤虫幼虫。在体外结果表明,添加 PirAB VP毒素显着调节了所研究弧菌属的毒力因子的产生。然而,这些体外结果无助于解释体内结果。因此,PirAB VP毒素似乎会加剧弧菌病。然而,相互作用的动力学是应变相关的。

更新日期:2020-09-02
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