Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details to perform heterotopic and partial orthotopic surgical models to promote ALS transplantation. After preservation and vessel cannulation steps, the liver grafts were decellularized. In addition, ex vivo blood perfusion tests were performed to obtain a successful anticoagulation treatment prior in vivo transplantation. Then, methods of partial liver resection, combination of hand-suture and cuff techniques to complete end-to-end anastomosis between the scaffold and the recipient animal were performed. These procedures which take 30–60 min and were efficient to allow acellular liver scaffold viability and recellularization of different types of cell post-surgery. In conclusion, our methods are practical and simple promising approach that provides the opportunity to investigate ways to achieve sufficient liver function post-transplantation in vivo.

无细胞肝支架 (ALS) 已成为移植的潜在候选者。到目前为止，所有涉及 ALS 移植的报告都在手术方法描述中失败，并且不支持科学家重现实验显微手术中使用的程序以使结果与文献相媲美。为了克服缺乏详细信息的问题，我们描述了执行异位和部分原位手术模型以促进 ALS 移植的手术步骤细节。在保存和血管插管步骤之后，肝移植物被去细胞化。此外，还进行了体外血液灌注测试，以获得成功的体内抗凝治疗移植。然后，采用部分肝切除、手工缝合和袖带技术相结合的方法完成支架和受体动物之间的端到端吻合。这些程序需要 30-60 分钟，并且有效地允许脱细胞肝支架存活和手术后不同类型细胞的再细胞化。总之，我们的方法是实用且简单的有前途的方法，它提供了研究在体内实现足够肝功能的方法的机会。