Abstract

This present study investigated the antioxidative and antidiabetic properties of kolaviron by analysing its inhibitory effect on key metabolic activities linked to T2D, in vitro and ex vivo. Kolaviron significantly inhibited α-glucosidase and α-amylase activities, and intestinal glucose absorption dose-dependently, while promoting muscle glucose uptake. Induction of oxidative pancreatic injury significantly depleted glutathione level, superoxide dismutase, catalase, and ATPase activities, while elevating malondialdehyde and nitric oxide levels, acetylcholinesterase and chymotrypsin activities. These levels and activities were significantly reversed in tissues treated with kolaviron. Kolaviron depleted oxidative-induced metabolites, with concomitant restoration of oxidative-depleted metabolites. It also inactivated oxidative-induced ascorbate and aldarate metabolism, pentose and glucuronate interconversions, fructose and mannose metabolism, amino sugar and nucleotide sugar metabolism, and arginine and proline metabolism, while reactivating selenocompound metabolism. These results depict the antidiabetic properties of kolaviron as indicated by its ability to attenuate oxidative-induced enzyme activities and dysregulated metabolisms, and modulated the enzyme activities linked to hyperglycaemia.

摘要

本研究通过分析 kolaviron 在体外和体外对与 T2D 相关的关键代谢活动的抑制作用，研究了 kolaviron 的抗氧化和抗糖尿病特性. Kolaviron 显着抑制 α-葡萄糖苷酶和 α-淀粉酶活性，以及​​剂量依赖性肠道葡萄糖吸收，同时促进肌肉葡萄糖摄取。氧化性胰腺损伤的诱导显着耗尽谷胱甘肽水平、超氧化物歧化酶、过氧化氢酶和 ATPase 活性，同时提高丙二醛和一氧化氮水平、乙酰胆碱酯酶和胰凝乳蛋白酶活性。这些水平和活动在用 kolaviron 处理的组织中显着逆转。Kolaviron 耗尽了氧化诱导的代谢物，同时恢复了氧化耗尽的代谢物。它还使氧化诱导的抗坏血酸和醛糖酸代谢、戊糖和葡萄糖醛酸相互转化、果糖和甘露糖代谢、氨基糖和核苷酸糖代谢以及精氨酸和脯氨酸代谢失活，同时重新激活硒化合物的代谢。这些结果描述了 kolaviron 的抗糖尿病特性，表现为它能够减弱氧化诱导的酶活性和代谢失调，并调节与高血糖相关的酶活性。