We appreciate the continued interest in our work and the extensive and constructive discussion provided by David Parks in his comment. We slightly disagree with the notion that the increments in sensitivity (Q) and the lower detection limit (DL) of spectral cytometry as compared to conventional cytometry, which we have described, are not significant. In our opinion, they are not only of statistical but also of operational significance. The experiments proposed by David Parks, aiming at a multivariate evaluation in situations of practical relevance, are well designed to underpin this pragmatic significance. In our original publication (1), we had already pointed out that the calculation of Q, B and DL based on the added values of the individual detection channels ignores the vector properties of the multispectral measurement. To account for this, we had introduced a multivariate overlap factor (O).

我们赞赏对工作的持续兴趣以及David Parks在其评论中进行的广泛而富有建设性的讨论。我们稍微不同意这样一种观念，即与我们所描述的传统细胞计数法相比，光谱细胞计数法的灵敏度（Q）和检测下限（DL）的增加并不明显。我们认为，它们不仅具有统计意义，而且具有业务意义。大卫·帕克斯（David Parks）提出的旨在针对实际相关情况下的多变量评估的实验，经过精心设计，可以支撑这种务实的意义。在我们的原始出版物中（1），我们已经指出，根据各个检测通道的相加值来计算Q，B和DL会忽略多光谱测量的矢量特性。为了解决这个问题，我们引入了一个多元重叠因子（O）。