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Salinity mediated enhancement in protein and pigment content in Spirulina (Arthrospira) platensis
Indian Journal of Biochemistry and Biophysics ( IF 1.5 ) Pub Date : 2020-08-17
G P W A Prabhath, S P Shukla, K Kumar, K K T Nuwansi

Spirulina (Arthrospira) platensis a filamentous multi cellular cyanobacterium utilized as animal feed and human food supplement. This cyanobacterium is also a rich source of value-added pigments with diverse applications in the food industry, pharmaceuticals and molecular biology. This report describes a procedure for enhancement of the yield of value-added pigment; c-phycocyanin (CPC) through the manipulation of salinity of the medium. The increase in salinity (sodium chloride-NaCl) from 0 to 3 g l-1 of the prescribed growth medium resulted in a considerable increase (11.83%) in CPC content. Growth of the organism and the purity of the CPC were not affected at elevated salt concentration. However, a further increase in the salinity (NaCl) from 3 to 7 g l-1 resulted in a decline in the content (26.67%) and purity (31.13%) of CPC. Considering the enhanced growth and CPC content through the manipulation of the concentration of the salt NaCl in the medium, it is concluded that a moderate amount of the salt (3 g l-1 NaCl) in the medium is a feasible and cost-effective approach for obtaining elevated protein biomass and enhanced the yield of the value-added pigment CCPS.

中文翻译:

盐度介导的螺旋藻蛋白和色素含量的增加

螺旋藻(Arthrospira)platensis一种螺旋状的多细胞蓝藻细菌,用作动物饲料和人类食品补充剂。这种蓝细菌也是丰富的增值颜料来源,在食品工业,制药和分子生物学中有多种应用。该报告描述了提高增值颜料产量的程序;c-藻蓝蛋白(CPC)通过操纵培养基的盐度。规定生长培养基的盐度(氯化钠-NaCl)从0增加到3 gl -1导致CPC含量显着增加(11.83%)。盐浓度升高不会影响生物的生长和CPC的纯度。但是,盐度(NaCl)从3 gl -1进一步增加导致CPC的含量(26.67%)和纯度(31.13%)下降。考虑到通过控制培养基中盐NaCl的浓度提高了生长和CPC含量,可以得出结论,培养基中适量的盐(3 gl -1 NaCl)是一种可行且经济高效的方法获得提高的蛋白质生物量并提高了增值颜料CCPS的产量。
更新日期:2020-08-17
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