A plasma separator with a multifunctional deformable chamber equipped with a porous membrane for point-of-care diagnostics.,Analyst

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A plasma separator with a multifunctional deformable chamber equipped with a porous membrane for point-of-care diagnostics.
Analyst ( IF 3.6 ) Pub Date : 2020-08-17 , DOI: 10.1039/d0an01014f
Xianbo Qiu ₁ , Huiqin Jiang , Xiaolei Zhang , Ke Li , Shengxiang Ge , Ningshao Xia , Michael G Mauk

Affiliation

Traditionally, plasma is extracted from whole blood using centrifuges in clinical laboratories, which is unsuitable for on-site testing. For point-of-care diagnostics, for example in HIV tests, to ensure the detection sensitivity for low-abundance analytical targets, a relatively large volume of plasma needs to be extracted from milliliters of blood with a simpler and easier-to-operate method than centrifugation. We report the development of a membrane-assisted, sedimentation-facilitated plasma separator with a multifunctional deformable chamber, which is able to perform plasma separation from undiluted whole blood in a short time. Multiple steps related to plasma separation, including cell sedimentation, cell filtration, and plasma driving and discharging, are all performed in or through the multifunctional deformable chamber equipped with a top-layer porous membrane, which significantly reduces the device complexity. Assisted by a simple jig or even hands, plasma separation can be conveniently performed upon mechanical actuation of the deformable chamber. Within 8 min, ∼130 μL of plasma can be conveniently extracted with the described device from 2.3 mL of whole blood. It has been demonstrated that HIV antibodies or virus spiked in whole blood can be successfully detected with reasonable sensitivity from the extracted plasma with the described pump-free device.

中文翻译：

一种带有多功能可变形腔室的血浆分离器，该腔室配备有用于现场诊断的多孔膜。

传统上，血浆是在临床实验室中使用离心机从全血中提取的，因此不适合现场测试。对于现场诊断（例如在HIV检测中），以确保对低丰度分析目标的检测灵敏度，需要使用一种更简单易操作的方法从毫升血液中提取相对大量的血浆比离心。我们报告了具有多功能可变形腔室的膜辅助，沉淀促进血浆分离器的发展，该腔室能够在短时间内从未稀释的全血中进行血浆分离。与血浆分离相关的多个步骤，包括细胞沉降，细胞过滤以及血浆驱动和放电，所有这些操作都在配备有顶层多孔膜的多功能可变形腔室中进行或通过多功能可变形腔室进行，这大大降低了设备的复杂性。在简单的夹具甚至双手的辅助下，可在机械驱动可变形腔室时方便地进行血浆分离。在8分钟内，可以使用上述装置方便地从2.3 mL全血中提取约130μL血浆。已经证明，使用所描述的无泵装置，可以从提取的血浆中以合理的灵敏度成功地检测出掺入全血中的HIV抗体或病毒。使用上述装置，可以方便地从2.3 mL全血中提取约130μL血浆。已经证明，使用所描述的无泵装置，可以从提取的血浆中以合理的灵敏度成功地检测出掺入全血中的HIV抗体或病毒。使用上述装置，可以方便地从2.3 mL全血中提取约130μL血浆。已经证明，使用所描述的无泵装置，可以从提取的血浆中以合理的灵敏度成功地检测出掺入全血中的HIV抗体或病毒。

更新日期：2020-09-14

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