Hypertrophic scar (HS) has been considered as a great concern for patients and a challenging problem for clinicians as it can cause functional debility, cosmetic disfigurement and psychological trauma. Although many methods have been developed to prevent and treat HS, the scarless healing is still a worldwide medical problem. In this study, palmatine-loaded poly(ε-caprolactone)/gelatin nanofibrous scaffolds (PCL/GE/PALs) were fabricated by electrospinning, and their effects on wound healing and HS formation were investigated. These nanofiber mats exhibit good antibacterial and antioxidant activities. In vitro studies indicate PCL/GE/PAL scaffolds can facilitate the adhesion, spreading and proliferation of L929 fibroblasts. In vivo tests demonstrate the full-thickness wounds treated with PCL/GE/PAL scaffolds heal about 3.5 days earlier than those in the control group. Scar elevation index measurements and histological analyses reveal PCL/GE/PAL scaffolds significantly inhibit HS formation, with the decrease in the thickness of dermis and epidermis, the number of fibroblasts, as well as the density of collagen and microvascular. Accelerating wound healing and inhibiting HS formation of these scaffolds are contributed to the sustained release of palmatine. The present work validates the potential use of palmatine-loaded electrospun nanofibrous scaffold PCL/GE/PALs as a functional wound dressing for healing wounds and preventing HS formation.

肥厚性瘢痕 (HS) 被认为是患者非常关心的问题，也是临床医生面临的一个具有挑战性的问题，因为它会导致功能衰弱、美容毁容和心理创伤。尽管已经开发了许多预防和治疗HS的方法，但无疤痕愈合仍然是一个世界性的医学问题。在这项研究中，通过静电纺丝制备了负载巴马汀的聚（ε-己内酯）/明胶纳米纤维支架（PCL/GE/PALs），并研究了它们对伤口愈合和HS形成的影响。这些纳米纤维垫具有良好的抗菌和抗氧化活性。体外研究表明 PCL/GE/PAL 支架可以促进 L929 成纤维细胞的粘附、扩散和增殖。体内测试表明，用 PCL/GE/PAL 支架治疗的全层伤口比对照组的伤口愈合时间早约 3.5 天。疤痕隆起指数测量和组织学分析表明，PCL/GE/PAL 支架显着抑制 HS 的形成，真皮和表皮的厚度、成纤维细胞的数量以及胶原蛋白和微血管的密度降低。加速伤口愈合和抑制这些支架的 HS 形成有助于巴马汀的持续释放。目前的工作验证了负载巴马汀的电纺纳米纤维支架 PCL/GE/PAL 作为功能性伤口敷料用于愈合伤口和防止 HS 形成的潜在用途。