Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region encoding a polyprotein of 2,480 amino acids. Further analysis revealed nt substitutions along the genome compared with that of the caprine kobuvirus reference strain, with 93% identity. Phylogenetic analysis indicated that the clade of the caprine kobuvirus was most closely related to porcine kobuviruses rather than bovine or ovine kobuviruses. Using primers designed from this genome, caprine kobuvirus was identified in the stools of other goats. Sanger sequencing of PCR products indicated 3D and VP1 gene nucleotides of this latter strain were 95% and 91% identical with those of MN604700, respectively. There were 35 and 101 nt substitutions in 3D and VP1 genes, respectively. Findings of kobuvirus over a 2-y period may indicate an endemic state, which needs further research. In addition, screening for kobuviruses over large geographic areas is needed to identify the evolutionary connections among different strains.

Kobuvirus 感染在人类、啮齿动物、食肉动物、猪和反刍动物中很常见。我们在此报告了来自明尼苏达州腹泻儿童的新型山羊科布病毒 (MN604700) 的完整基因组序列。全基因组测序显示 kobuvirus 基因组为 8,139 nt，具有编码 2,480 个氨基酸的多蛋白的单个 ORF 区域。进一步的分析揭示了与山羊科布病毒参考株相比，基因组中的 nt 替换，具有 93% 的同一性。系统发育分析表明，山羊科布病毒的进化枝与猪科布病毒的亲缘关系最密切，而不是牛科布病毒或绵羊科布病毒。使用从该基因组设计的引物，在其他山羊的粪便中鉴定出山羊科布病毒。PCR 产物的 Sanger 测序表明3D和VP1后一种菌株的基因核苷酸与 MN604700 的基因核苷酸分别有 95% 和 91% 的同一性。在3D和VP1基因中分别有 35 和 101 nt 替换。在 2 年期间发现 kobuvirus 可能表明一种地方病状态，这需要进一步研究。此外，需要在大的地理区域内筛选 kobuviruses，以确定不同毒株之间的进化联系。