This study was designed to optimize three microwave-assisted extraction (MAE) parameters (ethanol concentration, microwave power, and extraction time) of total phenolics, total flavonoids, and antioxidant activity of avocado seeds using response surface methodology (RSM). The predicted quadratic models were highly significant () for the responses studied. The extraction of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity was significantly () influenced by both microwave power and extraction time. The optimal conditions for simultaneous extraction of phenolic compounds and antioxidant activity were ethanol concentration of 58.3% (v/v), microwave power of 400 W, and extraction time of 4.8 min. Under these conditions, the experimental results agreed with the predicted values. MAE revealed clear advantages over the conventional solvent extraction (CSE) in terms of high extraction efficiency and antioxidant activity within the shortest extraction time. Furthermore, high-performance liquid chromatography (HPLC) analysis of optimized extract revealed the presence of 10 phenolic compounds, with rutin, catechin, and syringic acid being the dominant compounds. Consequently, this optimized MAE method has demonstrated a potential application for efficient extraction of polyphenolic antioxidants from avocado seeds in the nutraceutical industries.

中文翻译：

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使用响应面方法同时优化微波辅助提取酚类化合物和鳄梨 (Persea americana Mill.) 种子的抗氧化活性。


本研究旨在利用响应面法 (RSM) 优化牛油果种子总酚、总黄酮和抗氧化活性的三个微波辅助提取 (MAE) 参数（乙醇浓度、微波功率和提取时间）。预测的二次模型非常显着（ ）用于研究的反应。提取物中总酚含量（TPC）、总黄酮含量（TFC）和抗氧化活性显着（ ）受到微波功率和提取时间的影响。同时提取酚类化合物和抗氧化活性的最佳条件为乙醇浓度58.3%（v/v）、微波功率400 W、提取时间4.8 min。在这些条件下，实验结果与预测值一致。 MAE 与传统溶剂萃取 (CSE) 相比，在最短萃取时间内的高萃取效率和抗氧化活性方面显示出明显的优势。此外，优化提取物的高效液相色谱 (HPLC) 分析显示存在 10 种酚类化合物，其中芦丁、儿茶素和丁香酸是主要化合物。因此，这种优化的 MAE 方法已证明在营养保健品行业中从鳄梨种子中有效提取多酚抗氧化剂具有潜在的应用前景。