Pseudomonas aeruginosa is a Gram-negative bacterium that is commonly isolated from damp environments. It is also a major opportunistic pathogen, causing a wide range of problematic infections. The cell envelope of P. aeruginosa, comprising the cytoplasmic membrane, periplasmic space, peptidoglycan layer and outer membrane, is critical to the bacteria's ability to adapt and thrive in a wide range of environments. Over 40 proteases and peptidases are located in the P. aeruginosa cell envelope. These enzymes play many crucial roles. They are required for protein secretion out of the cytoplasm to the periplasm, outer membrane, cell surface or the environment; for protein quality control and removal of misfolded proteins; for controlling gene expression, allowing adaptation to environmental changes; for modification and remodelling of peptidoglycan; and for metabolism of small molecules. The key roles of cell envelope proteases in ensuring normal cell functioning have prompted the development of inhibitors targeting some of these enzymes as potential new anti-Pseudomonas therapies. In this review, we summarise the current state of knowledge across the breadth of P. aeruginosa cell envelope proteases and peptidases, with an emphasis on recent findings, and highlight likely future directions in their study.

中文翻译：

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铜绿假单胞菌的细胞包膜蛋白酶和肽酶：多种作用，多种机制。

铜绿假单胞菌是革兰氏阴性细菌，通常从潮湿环境中分离出来。它也是主要的机会病原体，引起各种问题感染。铜绿假单胞菌的细胞被膜，包括细胞质膜，周质空间，肽聚糖层和外膜，对细菌在各种环境中适应和繁殖的能力至关重要。铜绿假单胞菌中有40多种蛋白酶和肽酶细胞包膜。这些酶起着至关重要的作用。它们是蛋白质从细胞质分泌到周质，外膜，细胞表面或环境中所必需的；用于蛋白质质量控​​制和去除错误折叠的蛋白质；控制基因表达，适应环境变化；用于肽聚糖的修饰和重塑；和小分子的新陈代谢。细胞包膜蛋白酶在确保正常细胞功能中的关键作用促使人们开发了针对这些酶中某些酶的抑制剂，将其作为潜在的新型抗假单胞菌疗法。在这篇综述中，我们总结了铜绿假单胞菌广度的当前知识状态 细胞包膜蛋白酶和肽酶，重点是最近的发现，并突出了他们研究中可能的未来方向。